Position: Home > Articles > Development of Multiplex Taqman-LNA Real-time PCR For Detecting Pork,Chicken and Duck-Derived Ingredients in Meat Products
Chinese Journal of Veterinary Medicine
2017
(12)
81-84
应用多重Taqman-LNA荧光PCR同时检测肉制品中猪鸡鸭源性成分
作 者：
许如苏;纪玲珍;黄帅;周广彪;魏霜;段建发
单 位：
汕头出入境检验检疫局
关键词：
多重Taqman-LNA荧光PCR;猪源性成分;鸡源性成分;鸭源性成分;肉制品
摘 要：
为了建立同时检测肉制品中猪、鸡、鸭3种动物源性成分的多重Taqman-LNA荧光PCR,给打击肉制品掺假提供技术手段。针对动物线粒体基因组的保守序列,设计特异性引物和Taqman-LNA探针,建立同时检测肉制品中猪、鸡、鸭源性成分的多重荧光PCR方法,并应用于市售肉制品的检测。建立的多重Taqman-LNA荧光PCR方法可同时检测肉制品中上述动物源性成分,与牛、羊、驴、兔、鹅等动物源性成分无交叉反应,其检测限均达到肉含量的0.001%。对170份市售肉制品检测发现,有42份样品检出与标签标示成分不符的肉种成分,样品不合格率为24.7%。本研究建立的多重Taqman-LNA荧光PCR方法可同时检测肉制品中猪、鸡、鸭3种动物源性成分,具有特异、快速、经济、高效等优点,可适应于肉制品中多种肉源性成分的高通量检测。
译 名：
Development of Multiplex Taqman-LNA Real-time PCR For Detecting Pork,Chicken and Duck-Derived Ingredients in Meat Products
作 者：
XU Ru-su;JI Ling-zhen;HUANG Shuai;ZHOU Guang-biao;WEI Shuang;DUAN Jian-fa;Shantou Entry-Exit Inspection and Quarantine Bureau;
关键词：
Multiplex taqman-LNA PCR;;Pork-Derived Ingredients;;Chicken-Derived Ingredients;;duck-Derived Ingredients;;Meat Products
摘 要：
To develop a multiplex taqman-LNA real-time PCR for detecting pork,chicken and duck-Derived Ingredients in meat and products simultaneously. Species-specific primers and taqman-LNA probes were designed based on the COI gene sequences of the above animals. A multiplex taqman-LNA real-time PCR was developed for detecting the above animals-derived ingredients in meat and products after optimization of the reaction conditions. The assay was used to detect 170 samples of meat products from markets. The assay could detect 0. 001% each target meat of three meat from the above animals in meat products simultaneously,and had no cross-reaction with the DNA from cattle,sheep,donkey,rabbit,goose and other animals. 42 of 170 samples from markets were found to have some animal-derived ingredients that were not marked on the meat product labels. The assay was rapid,specific,economic and high-throughput to detect pork,chicken and duck-derived ingredients in meat products simultaneously.
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