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Position: Home > Articles > Study on the Metabolism of Carbohydrates in Cultures of Fritillaria ussuriensis During Different Stage of Tissue Culture Journal of Anhui Agricultural Sciences 2010,38 (8) 4397-4398

不同培养阶段的组培平贝母中碳水化合物代谢的研究

作  者:
于洋;朴炫春;孙丹;钱春荣;宫秀杰;廉美兰
单  位:
黑龙江省农业科学院耕作栽培研究所;长白山生物资源与功能分子教育部重点实验室(延边大学);黑龙江省农业科学院作物育种研究所
关键词:
平贝母;培养体;可溶性糖;还原糖;淀粉
摘  要:
[目的]进一步深入研究贝母器官发生机理,为今后分子水平上的研究提供参考。[方法]以平贝母为供试材料,以培养体的可溶性总糖含量、还原糖含量和淀粉含量为指标,研究了组培平贝母培养过程中培养体不同发育时期的碳水化合物代谢。[结果]培养体内可溶性总糖和还原糖含量按照组培平贝母培养体不同发育时期呈先降低后升高的趋势,到小鳞茎膨大时(小鳞茎-2),可溶性糖和还原糖的含量均达到最高,分别为53.41和9.23mg/g。不同阶段培养体内淀粉含量整体呈上升-下降-上升的趋势,在愈伤组织时期淀粉含量最低为42.32mg/g,而小鳞茎膨大时(小鳞茎-2)达到最高值为84.06mg/g。[结论]探明了碳水化合物代谢机制,为贝母器官发生机理提供理论依据。
译  名:
Study on the Metabolism of Carbohydrates in Cultures of Fritillaria ussuriensis During Different Stage of Tissue Culture
作  者:
YU Yang et al (Grop Cultivation Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)
关键词:
Fritillaria ussuriensis Maxim.;Cultures;Soluble sugar;Reducing sugar;Starch
摘  要:
[Objective] The study was to provide a reference for organ development and molecular study.[Method] Fritillaria ussuriensis Maxim.was used as an experiment material,total soluble sugar,reducing sugar and starch contents of tissue culture as indexes,the metabolism of carbohydrates in cultures of Fritillaria ussuriensis Maxim.during different stages of tissue culture were studied.[Result] The total soluble sugar and reducing sugar contents showed decrease and then increase trend in cultures of Fritillaria ussuriensis during different stages,and reached their maximum of 53.41 and 9.23 mg/g at a small bulblet expanding stage (bulblet-2).Total starch contents showed increase-decrease-increase trend in cultures.At callus stage reached their minimum of 42.32 mg/g,and reached maximum of 84.06 mg/g at a small bulblet expanding stage (bulblet-2).[Conclusion] The mechanism of the metabolism of carbohydrates was verified,which provided a theoretical basis for organ development.

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