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当前位置: 首页 > 文章 > 环境中氨基脲消解规律及对斑点叉尾鮰残留评估 农业环境科学学报 2013,32 (04) 26-33
Position: Home > Articles > Elimination Rules of the Semicarbazide in Environment and Assessment of Semicarbazide in the Channel Catfish(Ietalurus Punetaus)Tissue Journal of Agro-Environment Science 2013,32 (04) 26-33

环境中氨基脲消解规律及对斑点叉尾鮰残留评估

作  者：
索纹纹;刘永涛;艾晓辉;杨秋红;吕思阳
单  位：
华中农业大学水产学院;华中农业大学水产学院中国水产科学研究院长江水产研究所;中国水产科学研究院长江水产研究所
关键词：
养殖环境;斑点叉尾鮰;呋喃西林代谢物;氨基脲;消除;蓄积
摘  要：
为研究环境中呋喃西林代谢物氨基脲(Semicarbazide,SEM)在池塘和鱼体中的蓄积消解规律,采用全池泼洒呋喃西林的给药方式,采集环境(水样和底泥)和斑点叉尾鮰组织(皮肤和肌肉)样品,采样工作持续1年,并采用高效液相色谱串联质谱(HPLC-ESI/MS/MS)法对样品中SEM含量进行了分析。结果表明:水体中SEM初始阶段具有较高的消除速率,随后消除速率趋于平缓,并维持较长时间,底泥中SEM浓度随时间呈非线性下降;实验前5d,鱼皮中SEM出现了短暂蓄积后随时间呈线性下降,肌肉中SEM消除规律与水体中相似;水体、肌肉和鱼皮的消除半衰期T1/2分别为1.7、2.09、2.42d,水环境与斑点叉尾鮰组织中SEM浓度值存在显著相关性(P<0.01);至实验结束时,水体中和鱼体内氨基脲最终降至低于检出限(0.5μg·kg-1),底泥中氨基脲仍高于检出限;斑点叉尾鮰体内氨基脲含量与水环境密切相关,但鱼体中SEM并未出现明显富集。
译  名：
Elimination Rules of the Semicarbazide in Environment and Assessment of Semicarbazide in the Channel Catfish(Ietalurus Punetaus)Tissue
作  者：
SUO Wen-wen 1,2,LIU Yong-tao 2,AI Xiao-hui 2*,YANG Qiu-hong 2,L Si-yang 1,2,SHEN Dan-yi 3(1.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science,Freshwater Fish Germplasm Quality Supervision and Testing Center,Ministry of Agriculture,Wuhan 430223,China;3.Spreading Center of Technology in Aquatic Science of Cixi City in Zhejiang Province,Cixi 315300,China)
关键词：
aquaculture environment;channel catfish(Ietalurus Punetaus);nitrofurazone residues;semicarbazide SEM;eliminate;accumulation
摘  要：
Accumulation and elimination of semicarbazide(SEM)in channel catfish(Ietalurus Punetaus)tissue and pond under practical farming laboratory conditions were studied.We took the method of splashing nitrofurazone in whole pond,and the same sizes of channel catfish(124.13±25.2)g were exposed to freshwater containing semicarbazide for one year at natural conditions.The concentrations of SEM in environmenta(lwater,mud)and tissue(muscle,skin)samples was determined by liquid chromatography tandem mass spectrometric(HPLCESI/MS/MS).The results showed that semicarbazide concentration in water and tissue decreased quickly in the beginning,then the elimination trends started to level off and maintained a certain concentration fraction in certain period,but the semicarbazide concentration in mud presents downtrend in the overall period without a linear progression.The SEM in skin was enriched in the first five days,then the content of SEM represents decreased linearly over time.The elimination half-life of SEM in water,muscle and skin were 1.7 d,2.09 d and 2.42 d,respectively.Semicarbazide concentrations in channel catfish and water were below 0.5 μg·kg-1 at the end of the experiment except mud.The concentration in the channel catfish and the environment present significant correlation(P<0.01),but the SEM was not enriched obviously in the tissues of channel catfish.

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