Position: Home > Articles > Enzymatic Preparation of Chondroitin Sulfate from Swine Trachea
MEAT RESEARCH
2013,27
(12)
1-4
酶解法从猪气管中提取硫酸软骨素
作 者：
李鹏;时亚文;于辉;陈石良
单 位：
湖北奥彤生物制品科技有限公司
关键词：
猪气管;酶解;硫酸软骨素;工艺优化
摘 要：
以猪气管软骨为原料,利用二次酶解法提取硫酸软骨素,设计正交试验对酶解工艺进行优化,并测定了硫酸软骨素的纯度、含氮量、含氯化物量等主要指标。结果表明:最佳工艺组合条件为温度40℃,酶质量分数1.5%,提取时间2.5h,在此条件下平均得率达到10.98%,纯度达到85.22%。该工艺简便易行,有望为工业生产提供参考。
译 名：
Enzymatic Preparation of Chondroitin Sulfate from Swine Trachea
作 者：
LI Peng;SHI Ya-wen;YU Hui;CHEN Shi-liang;Hubei Ao-Tong Biological Science and Technology Co.Ltd.;
关键词：
swine trachea;;enzymatic hydrolysis;;chondroitin sulfate(CS);;process optimization
摘 要：
In this paper,the preparation of chondroitin sulfate(CS) by two-step enzymatic hydrolysis of swine trachea cartilage was optimized using orthogonal array design.The starting raw material was hydrolyzed sequentially by trypsin and a special-purpose enzyme preparation.The samples obtained were measured for purity and nitrogen and chloride contents.The optimal hydrolysis conditions were determined as 40 ℃,1.5% of enzyme concentration and 2.5 h.Under these conditions,the average yield of CS was 10.98% and the purity was 85.22%.The optimized process is simple and easy to operate and can provide useful evidence for future industrial production.
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