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当前位置: 首页 > 文章 > 螺旋粉虱成虫体内细菌群落多样性的PCR-DGGE和16S rRNA文库序列分析 昆虫学报 2012,55 (7) 12-21
Position: Home > Articles > Bacterial community in Aleurodicus dispersus (Hemiptera: Aleyrodidae) estimated by PCR-DGGE and 16S rRNA gene library analysis Acta Entomologica Sinica 2012,55 (7) 12-21

螺旋粉虱成虫体内细菌群落多样性的PCR-DGGE和16S rRNA文库序列分析

作  者：
王甸洪;吴伟坚;符悦冠
单  位：
华南农业大学昆虫生态研究室
关键词：
螺旋粉虱;总基因组DNA;16S rRNA文库;PCR-DGEE;系统发育;操作单元
摘  要：
为了解螺旋粉虱Aleurodicus dispersus体内细菌多样性和主要优势菌群结构,用PCR-DGGE和16S rRNA文库对采自于海南省番石榴上螺旋粉虱雌、雄成虫体内的细菌群落进行了分析。用PCR扩增体内细菌16S rRNA基因,构建雌、雄虫克隆文库;再用限制性片段长度多态性(restriction fragment length polymorphism,RFLP)方法从文库中筛选不同16S rRNA基因图谱,根据图谱对克隆子进行分型。从螺旋粉虱雌、雄两个样品中共获得10种分类操作单元(operational taxonomic unit,OTUs)。以16S rRNA基因为基础构建系统发育树,系统发育分析表明,螺旋粉虱雌、雄成虫体内优势菌群主要为发酵菌属Zymobacter,杀雄菌属Arsenophonus,泛菌属Pantoea和假单胞菌属Pseudomonas。Candidatus Portiera aleyrodidarum和Arsenophonus sp.可能为其体内共生菌群,在所有样品中均可稳定地检测到。这些微生物可能对螺旋粉虱生长发育、繁殖和性比调控起到重要的协同作用。
译  名：
Bacterial community in Aleurodicus dispersus (Hemiptera: Aleyrodidae) estimated by PCR-DGGE and 16S rRNA gene library analysis
作  者：
WANG Dian-Hong1, WU Wei-Jian1,*, FU Yue-Guan1,2 (1. Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China; 2. Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China)
关键词：
Aleurodicus dispersus; genomic DNA; 16S rRNA gene library; polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE); phylogenetic analysis; operational taxonomic unit (OTU)
摘  要：
To understand the bacterial diversity and the dominant types of bacteria in the spiraling whitefly, Aleurodicus dispersus Russell, bacterial communities present in both sexes of A. dispersus collected from Psidium guajava in Hainan were characterized using 16S rDNA-polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rRNA gene clone libraries. The partial bacterial 16S rRNA gene fragment was amplified with PCR, and the clone libraries were constructed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed by digestion of the 16S rRNA gene, and each unique restriction fragment polymorphism pattern was designated as an operational taxonomic unit (OTU). A total of 10 OTUs were identified from samples of both sexes of A. dispersus. Phylogenetic trees of bacterial 16S rRNA nucleotide sequences were constructed. Phylogenetic analysis revealed that Zymobacter, Arsenophonus, Pantoea, and Pseudomonas are the most dominant groups in both male and female adults of A. dispersus. Candidatus Portiera aleyrodidarum and Arsenophonus sp. , possibly the endosymbionts of A. dispersus, were detected in all samples. These bacteria may play synergetic roles in development, reproduction and sex-ratio control of the whitefly.

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