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当前位置: 首页 > 文章 > RSPO2和β-catenin在羊驼不同部位皮肤中表达差异的研究 畜牧兽医学报 2016,47 (5) 931-937
Position: Home > Articles > Different Expression of RSPO2 and β-catenin in the Different Sites of Alpaca Skins Chinese Journal of Animal and Veterinary Sciences 2016,47 (5) 931-937

RSPO2和β-catenin在羊驼不同部位皮肤中表达差异的研究

作  者：
高淑媛;于秀菊;程笳琪;宋亚军;赵兵令;牛姝;曹校瑞;赫晓燕
单  位：
山西农业大学动物科技学院;天水出入境检验检疫局
关键词：
羊驼;RSPO2;β-catenin;定位;表达差异
摘  要：
本研究旨在探讨RSPO_2和β-catenin对羊驼毛性状的作用。采用免疫组织化学、荧光定量PCR和Western blotting方法对RSPO_2和β-catenin在羊驼背部、腿部和耳部皮肤中基因和蛋白表达及蛋白定位进行研究。免疫组化结果显示:真皮乳头、毛囊基质、内根鞘、外根鞘、汗腺、表皮均可见RSPO_2的免疫阳性反应,并且主要出现在细胞质中,其中在内根鞘呈现强阳性;β-catenin在毛囊基质、内根鞘、外根鞘、汗腺、表皮内均出现免疫阳性反应。RT-PCR结果表明:RSPO_2在羊驼背部和腿部的表达量分别是耳部的7.633倍(P<0.05)和5.602倍(P<0.05),β-catenin在羊驼背部和腿部的表达量分别是耳部的3.689倍(P<0.05)和2.067倍(P>0.05)。Western blotting结果显示:羊驼皮肤提取物中存在与兔抗RSPO_2多克隆抗体和兔抗β-catenin多克隆抗体发生免疫阳性反应的蛋白条带,大小分别是26和86ku,羊驼皮肤平均蛋白表达量均为背部与耳部间差异显著(P<0.05),其中,RSPO_2在腿部与耳部表达量差异显著(P<0.05)。综上表明,RSPO_2和β-catenin与毛纤维的长度、弯曲度和细度呈正相关。
译  名：
Different Expression of RSPO2 and β-catenin in the Different Sites of Alpaca Skins
作  者：
GAO Shu-yuan;YU Xiu-ju;CHENG Jia-qi;SONG Ya-jun;ZHAO Bing-ling;NIU Shu;CAO Xiao-rui;HE Xiao-yan;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;Tianshui Exit Inspection and Quarantine;
关键词：
alpaca;;RSPO2;;β-catenin;;localization;;expression difference
摘  要：
Alpaca fiber is the ideal material for the textile industry.Its length,fineness and curvature are different in the back,leg and ear.In order to investigate the possible relationship between RSPO_2andβ-catenin with the characteristics of alpaca fleece,the expressions of RSPO_2andβ-cateninin back,leg and ear skins of alpaca were analyzed using immunohistochemistry,real-time PCR and Western blotting.The immunohistochemistry analysis demonstrated that the positive reaction of RSPO_2 was localized in the cytoplasmic of dermal papilla cells,hair follicle matrix,inner root sheath,outer root sheath,sweat glands and epidermis cells,in particular,the inner root sheath showed strongest positive reaction.The positive reaction ofβ-catenin was localized in hair follicle matrix,inner root sheath,outer root sheath,sweat glands and epidermis cells.Real-time PCR showed that the expression of RSPO_2 in back and leg skins were 7.633folds(P<0.05)and5.602folds(P<0.05),respectively,in comparison with that in ear skins.The expression ofβ-cateninin back and leg skins were 3.689folds(P<0.05)and 2.067folds(P>0.05),respective-ly,in comparison with that in ear skins.Western blot analysis showed that the positive band of RSPO_2(26ku)andβ-catenin(86ku)were strongest in back skins and weakest in ear skins with significant difference(P<0.05).The positive band of RSPO_2 in leg skins was significantly stronger than that in ear skins(P<0.05).The results suggested that RSPO_2andβ-catenin were positively associated with regulating the length,curvature and fineness formation of alpaca fleece.

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