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当前位置: 首页 > 文章 > 猪粪尿中添加不同底物对挥发性脂肪酸和本土菌群的影响研究 农业环境科学学报 2009,28 (02) 388-392
Position: Home > Articles > Effect of Adding Different Substrates to Swine Waste Slurries on Volatile Fatty Acids and Indigenous Bacterial Genera Journal of Agro-Environment Science 2009,28 (02) 388-392

猪粪尿中添加不同底物对挥发性脂肪酸和本土菌群的影响研究

作  者：
黄灿;唐新燕;彭绪亚;李季
单  位：
重庆大学生物工程学院;重庆大学三峡库区生态环境教育部重点实验室;中国农业大学资源与环境学院
关键词：
底物;猪粪尿混合物;挥发性脂肪酸;本土菌群
摘  要：
猪粪尿中含有一些未经消化的有机物质,如蛋白质、淀粉,在厌氧或兼性厌氧条件下,粪尿中的微生物能利用这些有机质作为代谢底物,产生挥发性脂肪酸、醇类、胺类、芳香类等恶臭化合物,然而很少有研究比较不同底物在粪尿恶臭产生过程中的作用。本研究采用室内培养方法,将收集的新鲜猪粪、尿和去离子水按10:7:3的比例(重量比)进行混合摇匀配成猪排泄物样本,旨在探讨蛋白质、淀粉等不同底物在粪尿挥发性脂肪酸等恶臭化合物产生过程中的作用及其对粪尿本土菌群的影响。结果表明,玉米淀粉、酪蛋白的添加均促进了猪粪尿中总挥发性脂肪酸的产生量,5周后未添加的对照组、添加玉米淀粉、酪蛋白组总挥发性脂肪酸浓度分别由培养初期的60mmol.L-1增加至5周后的133、183、172mmol.L-1。就粪尿中总挥发性脂肪酸的具体组分而言,酪蛋白的添加增加了异丁酸、戊酸、异戊酸等长链或分支的挥发性脂肪酸在猪粪尿总挥发性脂肪酸中的比例,5周内异丁酸、戊酸、异戊酸占总挥发性脂肪酸比例分别由培养前的1.4%、7.4%、5.6%增加至5周后的13.9%、10%、14%。本土菌群中,玉米淀粉、酪蛋白的添加促进了猪粪尿中乳酸菌、优杆菌的增殖。玉米淀粉、酪蛋白的添加对链球菌、梭菌作用效果并不相同,玉米淀粉更易于链球菌的生长,而酪蛋白更易于梭菌的生长。
译  名：
Effect of Adding Different Substrates to Swine Waste Slurries on Volatile Fatty Acids and Indigenous Bacterial Genera
作  者：
HUANG Can1,TANG Xin-yan2,PENG Xu-ya1,LI Ji3(1.Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment,Ministry of Education,Chongqing University,Chongqing 400045,China;2.College of Bioengineering,Chongqing University,Chongqing 400044,China;3.College of Resource and Environment Sci-ence,China Agriculture University,Beijing 100094,China)
关键词：
substrates;swine waste slurries;volatile fatty acids;indigenous bacterial genera
摘  要：
Microorganisms play an important role in the production of odors in swine waste slurries.It has accessed to a wide variety of unidi-gested swine waste slurries substances,including lipids,protein and carbohydrates to produce a complex chemical mixture of volatile fatty acids(VFAs),alcohols,amides and aromatic compounds.It is proved that VFAs most closely correlate to manure odor.However,no informa-tion is available on the effect of different manure substances on VFAs production in vitro.The objective of this study was to assess in vitro the effect of different manure substances on total VFAs,individual VFAs and main indigenous bacterial genera in swine waste slurries.After col-lection,swine waste slurry(faeces:urine:deionized water,10:7:3,weight basis)were blended.The treatments used for this study were no ad-dition control,5%(m/m)corn starch and 5%(m/m)casein.The results indicated that the total VFAs in all treatments continuously increased throughout the 5 weeks of the incubation.After the 5-week incubation,the content of total VFAs in no addition control,corn strach and casein group increased from an initial average concentration of 60 mmol.L-1 to 133 mmol.L-1,183 mmol.L-1and 172 mmol.L-1 respectively.As for individual VFAs,addition of casein had a substantial impact on the accumulation of isobutyrate,valerate and isovalerate and percentages of isobutyrate,valerate and isovalerate relative to total VFA concentration in swine waste slurry increased from an initial 1.4%,7.4% and 5.6% to 13.9%,10% and 14% respectively after the 5-week incubation.Addition of different substances in vitro to swine waste slurry had different impact on different indigenous bacterial genera.Addition of both corn starch and casein increased the number of Lactobacillus and Eubacteri-um in swine waste slurries.Compared with others indigenous bacterial genera,corn starch was more beneficial to the growth of Streptococcus.In contrast,Clostridium was prone to use casein.

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