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Position: Home > Articles > Polymorphisms of Growth Hormone Gene in Xingyi Bantom China Animal Husbandry & Veterinary Medicine 2010,37 (12) 98-101

兴义矮脚鸡GH基因的多态性研究

作  者:
朱丽莉;王闯;傅筑荫;陈彬;张福平
单  位:
贵州大学动物科学学院贵州省动物遗传育种与繁殖重点实验室
关键词:
兴义矮脚鸡;GH基因;多态性
摘  要:
兴义矮脚鸡为贵州特有的地方品种鸡。本研究对兴义矮脚鸡GH基因上第一外显子和部分第一内含子序列直接测序进行多态性分析。结果表明,在扩增出来的777 bp序列中,A、T、G、C碱基的平均含量分别为28.1%、23.9%、24.9%和23.1%。A+T的含量(52%)略高于G+C的含量(48%);总共发现10个变异位点,占分析位点总数的1.287%,这些变异位点分别为C124T、T319G、T321C、G333A、A364G、A464C、A507C、C508A、T541C和T630C,其中C124T突变点位于5′端调控区,其余突变点都集中在第一内含子上,均为转换,表现较高的转换偏向。
译  名:
Polymorphisms of Growth Hormone Gene in Xingyi Bantom
作  者:
ZHU Li-li,WANG Chuang,FU Zhu-yin,CHEN Bin,ZHANG Fu-ping(College of Animal Science,Guizhou University,Guizhou Key Laboratory of Animal Genetics Breeding and Reproduction,Guiyang,550025,China)
关键词:
Xingyi bantom;GH gene;polymorphism
摘  要:
Xingyi bantom is a special Guizhou chicken breed native.In the present study,the polymorphism of exon 1 and inton1 of growth hormone(GH) gene about 777 bp was measured with the method of direct sequenceing in Xingyi bantom.The results showed that the complete sequences was A28.1%,T23.9%,G24.9% and C23.1% respectively.A+T(52%)was higher than G+C(48%).A total of 10 were detected that covering 1.287% of the entire length of the sequence.The mutation sites were C124T,T319G,T321C,G333A,A364G,A464C,A507C,C508A,T541C and T630C.Meanwhile the C124T in 5′-UTR and the others in intron 1.All of them are transformation phenotyping the higher the deviation.

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