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当前位置: 首页 > 文章 > 牛奶中多种磺胺类抗生素残留的HPLC快速分析 食品科学 2007,28 (6) 256-259
Position: Home > Articles > Analysis of Sulfonamide Residues in Milk with Rapid HPLC Method FOOD SCIENCE 2007,28 (6) 256-259

牛奶中多种磺胺类抗生素残留的HPLC快速分析

作  者：
孙晶玮;赵新淮
单  位：
东北农业大学乳品科学教育部重点实验室
关键词：
磺胺;牛奶;分析;反相高效液相色谱
摘  要：
研究了牛奶中磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲基嘧啶、磺胺甲噁唑、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶六种磺胺类抗生素的多残留高效液相色谱(HPLC)分析方法。样品经氯仿-丙酮(2:1,V/V)提取两次,提取液浓缩至干。残渣用流动相甲醇-0.08%乙酸(60:40,V/V)溶解,净化后以反相C18柱,甲醇-0.08%乙酸等度洗脱,紫外检测器检测、外标法定量。结果表明,这六种抗生素在0.01～1.0mg/L浓度范围内呈线性关系,磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲基嘧啶检的检出限为0.010mg/L、磺胺甲噁唑、磺胺间甲氧嘧啶、磺胺间二甲氧嘧啶检出限分别为0.015mg/L。分析的添加回收率为69.4%～123.5%,变异系数为<8%,整体分析时间小于3h。方法具有处理简单、检测快速、准确的特点。
译  名：
Analysis of Sulfonamide Residues in Milk with Rapid HPLC Method
作  者：
SUN Jing-wei,ZHAO Xin-huai (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China)
关键词：
sulfonamide;milk;analysis;RP-HPLC
摘  要：
The analysis method on six sulfonamides in milk, namely sulfamerazine, sulfamethazine, sulfamethoxazole, sulfamonomethoxine, sulfadimethoxine and sulfadiazine was studied. Milk containing sulfonamides was extracted with chloroform -acetone mixture (2:1, V/V) twice. The extract was combined and evaporated to dry, then dissolved in methanol-0.08% acetic acid (60:40, V/V). Purified by hexane, the extract was analyzed in HPLC in a C18 column with methanol-acetic acid-H2O as eluent. Sulfonamide residues were detected by a UV detector and their concentrations were determined with calibrated curves. A good linear relationship exists for all residues in concentration range of 0.01～1.0 mg/L, while the detection limit for sulfadiazine and sulfamerazine and sulfamethazine is 0.010 mg/L and for sulfadimethoxine and sulfamonomethoxine and sulfadimethoxine 0.015 mg/L. The analysis procedure needs total time less than 3 h with an addition recovery for sulfonamides about 69.4%～ 123.5% and coefficient of variation less than 8%. The method is rapid, simple and accurate.

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