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Position: Home > Articles > Screening and Analysis of the Genes Whose Expressions are Up-regulated by Abiotic Stresses in Chloris virgata Molecular Plant Breeding 2008,6 (5) 70-76

盐胁迫下虎尾草上调基因的筛选和鉴定

作  者：
梁涵;张欣欣;管清杰;罗秋香;柳参奎
单  位：
东北林业大学盐碱地生物资源环境研究中心
关键词：
虎尾草;ESTs;基因表达;胁迫;上调
摘  要：
虎尾草(Chloris virgata)是禾本科虎尾草亚族的一种一年生草本植物,适应性极强,能在恶劣的盐碱化土壤上生长。本实验构建了虎尾草的cDNA基因文库,随机挑选出2300个克隆,并且通过点杂交分析在NaCl胁迫下这些基因的表达情况。结果表明有59个基因在NaCl胁迫下强烈表达,这些上调基因中23个同时也被NaHCO3胁迫表达。这个结果暗示了NaCl逆境与NaHCO3逆境在基因表达的应答机制存在明显的差异。59个基因可以分成8类:能量代谢(21.7%),信号转导(5.0%),氧化逆境(13.3%),光合作用(10.0%),生长发育(5.5%),转录因子(1.67%),各种酶类(10.0%)和一些未知基因(28.3%)。从中选出了一些上调基因进行Northern杂交分析,Northern杂交表明这些基因中的有些是在根或叶中表达,与点杂交的结果有一致的趋势。
译  名：
Screening and Analysis of the Genes Whose Expressions are Up-regulated by Abiotic Stresses in Chloris virgata
作  者：
Liang Han Zhang Xinxin Guan Qingjie Luo Qiuxiang Liu Shenkui Alkali Soil Natural Environmental Science Center(ASNESC),Northeast Forestry University,Harbin,150040
关键词：
Chloris virgata,ESTs,Gene expression,Stresses,Up-regulation
摘  要：
Chloris virgata(feather fingergrass),is an one-year-old herbaceous plant of Gramineae.It has high adaptability and can survive in highly saline soils.We randomly selected 2 300 clones from a C.virgata cDNA library and examined their expressions under NaCl stress by Dot blot analysis.About 59 EST clones were strongly expressed under NaCl stress.Twenty-three of these up-regulated genes was also induced by NaHCO3.This result suggests that it has notable differences in response mechanism of gene expression under NaCl and NaHCO3.The ESTs were classified into eight groups:energy metabolism(21.7%),signal transduction(5.0%),oxidation adversity(13.3%),photosynthesis(10.0%) and growth development represented(5.5%),transcription factors(1.67%),enzymes(10.0%) and unknown genes(28.3%).Some up-regulated genes were selected for Northern blot.The Northern blot proved that some up-regulated genes expressed in roots,some in leaves,and others in both of them.The result was the same with Dot blot.

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