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MiRNAs在番茄钾营养胁迫中的作用

作  者:
赵晓明;张宁;安欣悦
单  位:
吉林农业科技学院农学院
关键词:
番茄;钾素胁迫;miRNAs;靶基因
摘  要:
以低钾不同耐性的2种基因型番茄(JZ18-钾敏感型和JZ34-耐低钾型)为试材,采用新一代高通量测序技术,对低钾胁迫下2种不同基因型番茄根系miRNAs进行测序,根据检测结果进行生物信息学分析,以期为研究响应低钾胁迫的关键miRNA提供参考依据。结果表明:低钾胁迫下,2个基因型番茄根系中表达差异显著,且表达趋势相反的miRNAs包括miR167b、miR167g-5p、miR168a、miR171b。对其基因表达进行荧光实时定量(qRT-PCR)验证,并进一步对靶基因进行预测分析与功能研究,明确miR168a与其靶基因SlAGO1存在负调控关系并参与调控番茄的低钾胁迫响应。
译  名:
Role of miRNAs in Tomato Under Potassium Deficiency Stress
作  者:
ZHAO Xiaoming;ZHANG Ning;AN Xinyue;College of Agronomy,Jilin Agriculture Science and Technology University;College of Horticulture,Shenyang Agricultural University;
关键词:
tomato;;K+-deficiency stress;;miRNAs;;target gene
摘  要:
High-throughput sequencing was performed using two tomato genotypes root(low-K~+ sensitive JZ18 and low-K+tolerant JZ34),and bioinformatics analysis was carried out according to the test results,in order to provide reference for studying key miRNAs in response to K+-deficiency stress.The results showed that the miRNAs(miR167 b,miR167 g-5 p,miR168 a,miR171 b)were significantly expression and opposite expressed in the roots of the two genotypes under K+-deficiency stress.The gene expression was verified by real-time quantity(qRT-PCR),and the target gene was further predicted and functionally studied to confirm that miR168 a had a negative regulatory relationship with its target gene SlAGO1,and was involved in the regulation of K+-deficiency stress response in tomato.

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