关键词：
水稻;SSR标记;分子定位;偏态分离;特异亲和基因;杂种不育性
摘 要：
水稻籼粳亚种间杂种具有强大的优势 ,但亚种间杂种的不育性限制了这一优势的利用。开展杂种不育基因的定位工作 ,对于进一步了解杂种不育性的遗传基础 ,克服亚种间杂种的不育性具有重要的意义。本研究选用粳型品种台中 6 5的近等基因系E4 7- 1和籼型品种广陆矮 4号为材料 ,利用 74个SSR标记对杂种F2 群体进行偏态分离标记的筛选 ,同时根据F2 和F3群体花粉育性和具有偏态分离的SSR标记之间的连锁关系 ,对特异亲和基因 (F1花粉不育基因 )S e座位进行了分子定位 ,取得了以下主要结果 :1、利用 116个均匀分布在水稻 12条染色体上的SSR标记对籼粳两亲本进行多态性筛选。结果有10 1个SSR标记在亲本间具有多态性 ,15个SSR标记在亲本间无多态性 ,SSR标记在亲本间的多态率高达 87 0 7%。2、选用 74个亲本间具有多态性的SSR标记对E4 7- 1/广陆矮 4号组合F2 群体的偏态分离进行了初步的筛选和分析。发现有 6个染色体区段的 9个SSR标记在F2 群体中存在偏态分离 ,它们分别位于第3、第 6、第 7、第 10、第 11和第 12染色体上 ,卡方值均达到显著或极显著水平。 6个染色体区段中有 2个严重偏态分离区段 ,分别位于第 6和第 12染色体。3、通过对F2 群体的花粉育性和偏态分离区段的SSR标记基因型的相关关系分析 ,表明位于第
译 名：
Mapping of the Specific Compatibility Gene S-e using Molecular Markers in Rice(Oryza sativa L.)
作 者：
Postgraduate: Mr Zhu W Y Superviser:Dr & Prof Zhang G Q South China Agricultural University, Guangzhou, 510642
关键词：
Rice, SSR markers, Molecular mapping; Distorted segregation, Specific compatibility gene, Hybrid sterility
摘 要：
There is strong heterosis in F 1 hybrids between indica and japonica subspecies of the cultivated rice ( Oryza sativa L ), but high sterility in F 1 hybrids hampered the utilization of the heterosis Therefore, to overcome this sterility is very important for turning the hybrid vigor into yield vigor. According to Specific Compatibility Hypothesis, F 1 pollen sterility is the main form of hybrid sterility. Hence, mapping sterility genes is important for understanding the genetic basis of F 1 sterility. In this study, indica variety D2 and japonica variety E47-1 were used as parents to construct F 2 and F 3 populations. Seventy four SSR markers were used to detect the distorted segregation in F 2 population. On the basis of the correlation between pollen fertility of F 2 population and distorted segregation markers, different genotypic plants were selected to develop different F 3 populations. Using one F 3 population, S e locus was mapped on chromosome 12. The main results of the study are as follows: 1 One hundred and sixteen SSR markers were used to detect the polymorphism between parents. One hundred and one SSR markers were found to have the polymorphism between the two parents, whereas fifteen SSR markers did not show any polymorphism. The rate of polymorphism of SSR markers was 87 07%. 2 In order to find out chromosomal regions having distorted segregation, seventy four SSR markers distributed over the whole rice genome were selected. Nine distorted segregation markers located on chromosome 3, 6, 7, 10,11 and 12 were found in F 2 segregating population. Two distorted segregation chromosomal regions, which maybe caused by male sterility genes were found on chromosome 6 and chromosome 12. 3 Analysis of the correlation between pollen fertility and distorted segregation SSR markers showed that F 1 pollen sterility gene S e was located near RM19 marker on chromosome 12. Subsequently, collecting a single plant heterozygous on the chromosomal region near RM19 marker, F 3 mapping population was developed. Designing some new SSR markers near RM19 and using the F 3 mapping population, S e locus was mapped. The genetic distance between the gene and the flanking markers PSM401, PSM180, PSM182 and RM19 were 2 3cM, 1 3cM, 3 7cM and 4 3cM, respectively. 4 Another F 3 population, developed from a single pollen sterility plant with five homozygous loci S a, S b, S c, S d, S e was studied. Through the analysis of the pollen fertility in F 3 population, another F 1 pollen sterility gene S f was reconfirmed. Mapping F 1 pollen sterility gene S e is important to enrich and perfect Specific Compatibility Hypothesis. It can also provide the strong basis for breeding indica compatible japonica lines with molecular marker assisted selection.
