当前位置: 首页 > 文章 > 单酶法RTP-CR检测马铃薯纺锤块茎类病毒 华北农学报 2002,17 (1) 41-45
Position: Home > Articles > Detection of Potato Spindle Tuber Viroid by Single-enzyme RT-PCR Acta Agriculturae Boreali-Sinica 2002,17 (1) 41-45

单酶法RTP-CR检测马铃薯纺锤块茎类病毒

作  者:
叶明;盛长忠;石明;安春菊;冀俊丽;吴学锋;杜荣骞;李德森
单  位:
南开大学生命科学学院
关键词:
马铃薯纺锤块茎类病毒;单酶法RTPCR;Taq酶
摘  要:
根据Taq酶既有聚合酶活性又有反转录酶活性的特点 ,探索了只在Taq酶单独作用下完成RT PCR反应的条件 ,以达到对马铃薯纺锤块茎类病毒 (PSTV)的快速检测。结果表明 ,反转录阶段退火温度在 37℃、循环数不少于 10次的情况下 ,PCR扩增能得到一条相关特异带。经过嵌合式PCR证明 ,这条带为PSTV的特异带。与其他检测方法相比 ,用单酶法RT PCR检测PSTV步骤简单 ,成本低廉
译  名:
Detection of Potato Spindle Tuber Viroid by Single-enzyme RT-PCR
作  者:
YE Ming,SHENG Chang zhong,SHI Ming,AN Chun ju, JI Jun li,WU Xue feng,DU Rong qian,LI De sen (College of Life Sciences,Nankai University,Tianjin 300071,China)
关键词:
PSTV;Single enzyme RT PCR;Taq enzyme
摘  要:
In single enzyme RT PCR,Taq enzyme plays as roles of both reverse transcripase and DNA polymerase.The reaction conditions of single enzyme RT PCR was researched in this paper in order to detect PSTV rapidly.The results indicated that when the anneal temperature was 37 ℃ and the circles were more than 10 times in reverse transcription phase,a relative band of PSTV was obtained by PCR which could be proved using nested set PCR.Single enzyme RT PCR was simple and low cost,so it could play on important role for the detection of PSTV.

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