当前位置: 首页 > 文章 > 不同生态区罂粟种质的遗传多样性ISSR分析 植物遗传资源学报 2012,13 (2) 239-243
Position: Home > Articles > ISSR Genetic Diversity of Opium Poppy Germplasms in Different Ecological Zones Journal of Plant Genetic Resources 2012,13 (2) 239-243

不同生态区罂粟种质的遗传多样性ISSR分析

作  者:
魏玉杰;张金文;何庆祥;陈永坤;陈凌娜
单  位:
甘肃亚盛集团博士后科研工作站;甘肃农业大学;甘肃省农垦农业研究院
关键词:
罂粟;ISSR;遗传多样性
摘  要:
为了揭示罂粟种质的遗传多样性,利用ISSR分子标记进行遗传多样性研究,从100条随机引物中筛选出9条多态性引物对所有样品扩增,共检测到109个等位变异位点,引物等位位点数在9~16之间,平均每条引物有12个等位变异,其中,引物807的等位位点最多,为16个。引物等位位点多态性信息量PIC值为0.77~0.92。其中引物807的PIC值最大(0.92),引物847的PIC值最小(0.77),遗传相似系数在-0.452~0.981之间,且明显聚为2个类群;亲缘关系和地理分布呈一定的相关性,但没有形成明显的地理变异模式;本研究将为罂粟种质资源科学有效利用提供理论依据。
译  名:
ISSR Genetic Diversity of Opium Poppy Germplasms in Different Ecological Zones
作  者:
WEI Yu-jie 1,2,ZHANG Jin-wen1,HE Qing-xiang2,CHEN Yong-kun3,CHEN Ling-na3(1 Gansu Agricultural University,Lanzhou 730070;2Gansu State Farms Academy of Agricultural Researches,Wuwei 733006;3Postdoctoral Scientific Research Station of Gansu Yasheng Group,Beijing 100101)
关键词:
Opium poppy;ISSR;Genetic diversity
摘  要:
In order to reveal the genetic diversity of opium poppy in different ecological zones,based on the molecular markers of 100 inter-simple sequence repeat(ISSR),poppy and relatives from 12 different areas were amplified and analyzed with optimizing conditions for the primers.The results showed that a total of 109 allelic variations were detected,between 9 and 16 loci for each primer.Among them,primer 807 had the most alleles of 16.The value of allelic polymorphism information content(PIC)ranged from 0.77 to 0.92,which the Maximum is 807(0.92)and the minimum is 847(0.77).Genetic similarity among them ranged from-0.452 to 0.981,and these 12 germplasms were classified into 2 groups.The poppy germplasms sources was less correlated with geographic latitude.There was no evident geographic patterns of the poppy germplasm sources happened.The research will provide basis theoretical for scientific and effective utilization poppy germplasms.

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