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当前位置: 首页 > 文章 > 不同质量浓度的蔗糖和硼酸对北京杨花粉萌发的影响 吉林林业科技 2012,41 (5) 1-4
Position: Home > Articles > The effect of sucrose and boric acid on the pollen germination of Populus×beijingensis Journal of Jilin Forestry Science and Technology 2012,41 (5) 1-4

不同质量浓度的蔗糖和硼酸对北京杨花粉萌发的影响

作  者：
李永生;耿淑艳;王永和
单  位：
长春大学生物科学技术学院;上营森林经营局
关键词：
北京杨;花粉萌发;蔗糖;硼酸;质量浓度
摘  要：
以北京杨新鲜花粉为材料,研究固体培养基中蔗糖和硼酸不同质量浓度对花粉萌发的影响,结果表明:在培养基不含硼酸时,最适宜萌发的蔗糖质量浓度为10%,萌发率93.5%;当达到20%时,抑制花粉萌发,萌发率仅为55.6%。在培养基不含蔗糖时,最适宜萌发的硼酸质量浓度为0.01%,随着质量浓度升高,萌发率呈下降状态。在蔗糖和硼酸的双重作用下,最适宜萌发的质量浓度为10%蔗糖+0.01%硼酸,萌发率高达98.6%,花粉管生长快、健壮。新鲜花粉生活力测定的培养时间为24 h。
译  名：
The effect of sucrose and boric acid on the pollen germination of Populus×beijingensis
作  者：
LI Yong-sheng1,GENG Shu-yan2,WANG Yong-he1(1.Biotechnology College of Changchun University,Changchun 130012,China;2.Shangying Forest Management Bureau of Shulan,Jilin 132607,China)
关键词：
Populus × beijingensis;pollen germination;sucrose;boric acid;mass concentration
摘  要：
The effect of sucrose and boric acid with different concentration in solid medium on the pollen germination of Populus × beijingensis were studied in this paper.Results showed that without the boric acid,the optimal concentration of sucrose was 10%,and the pollen germination rated was 93.5%.The pollen germination was only 55.6% if the concentration of sucrose was 20%.Without sucrose,the optimal concentration of boric acid was 0.01%.The pollen germination rate decreased with the increasing concentration of boric acid.With both sucrose and boric acid,the optimal medium was with 10% sucrose and 0.01% boric acid.Under this condition,the pollen germination rate was 98.6%,and the pollen tube grew fast and healthy.The cultivating time for measuring viability of fresh pollen was 24 h.

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