Construction of 1S~1 Chromosome-specific Molecular Markers in Aegilops longissima and Its Application

LIU Xiao-ming;ZHANG Shu-qian;GONG Wen-ying;TANG Hai-tian;WANG Can-guo;CHENG Dun-gong;LIU Cheng;LIU Jian-jun;Research and Development Center of Biotechnology,Weifang University of Science and Technology;School of Chemistry and Environment,Weifang University of Science and Technology;Crop Research Institute, Shandong Academy of Agricultural Sciences;Yantai Oceanic Environmental Monitoring Central Station;

Aegilops longissima;;chromosome 1Sl;;molecular marker;;wheat breeding

Since the wheat introduced Aegilops longissima 1Slchromosome had significantly higher processing quality and higher content of iron and zinc in grain,the construction Aegilops longissima 1Sl-specific molecular markers was necessary and important for selecting and breeding high quality wheat which with high iron and zinc content. In this research,9 molecular markers specific for Aegilops longissima 1Slwere constructed,in which,2 markers located on the short arm,6 markers located on the long arm,and 1 marker located on both short and long arms.The verification experiment showed that the 9 markers were very available in screening and identifying isolated population.It indicated that the constructed 9 Aegilops longissima 1Slchromosome-specific molecular markers could be used in cross population screening,identification and high quality wheat breeding.