Position: Home > Articles > Fingerprint Profiling of Virgara Shiraz Wine by High Performance Liquid Chromatography-Ion Trap Mass Spectrometry
FOOD SCIENCE
2014,35
(12)
137-142
进口西拉葡萄酒的HPLC-DAD-MS特征指纹图谱
作 者：
张朋杰;张宪臣;王勇;杨芳;李双宜;李蓉;华洪波;胡仪光;卢俊文
单 位：
中山市广东外语外贸大学附设外语学校;中山出入境检验检疫局
关键词：
高效液相色谱-二极管-离子阱质谱;指纹图谱;相似度;进口葡萄酒;质量评价
摘 要：
建立进口西拉葡萄酒的高效液相色谱-二极管-离子阱质谱指纹图谱分析方法。样品采用乙酸乙酯萃取,旋转蒸发仪浓缩,甲醇溶解,高效液相色谱-离子阱质谱分析测定。色谱柱采用MGⅡC18柱(150 mm×2.0 mm,5μm),以pH 3.0水-乙腈为流动相,梯度洗脱,流速0.25 mL/min,多波长检测,离子阱质谱采用负离子模式,扫描范围m/z 50~1 200,可对没食子酸等11个组分进行定性和定量。维拉西拉葡萄酒的15个共有峰在60 min内得到良好分离,通过与标准品比较,对其中6个峰进行了确证,10批次维拉西拉葡萄酒的相似度均大于0.9。方法简便、灵敏、稳定、精确,生成的葡萄酒指纹图谱能够用于进口西拉葡萄酒的质量评价。
译 名：
Fingerprint Profiling of Virgara Shiraz Wine by High Performance Liquid Chromatography-Ion Trap Mass Spectrometry
作 者：
ZHANG Peng-jie;ZHANG Xian-chen;WANG Yong;YANG Fang;LI Shuang-yi;LI Rong;HUA Hong-bo;HU Yi-guang;LU Jun-wen;Entry-Exit Inspection and Quarantine Bureau;Zhongshan Foreign Language School Attached to Guangdong University of Foreign Studies;
关键词：
high perfomance liquid chromatography-diode array detector-ion trap mass spectromtry(HPLC-DAD-MS);;chromatographic fingerprint;;similarity;;import wine;;quality evaluation
摘 要：
A chromatographic fingerprint analysis method for Australian Virgara Shiraz wine was established by high performance liquid chromatography-diode array detector-ion trap mass spectrometry(HPLC-DAD-MS). Samples were extracted with acetic ether, concentrated with a rotary evaporator, and re-dissolved with methanol. HPLC-MS separation was performed on a MG Ⅱ C18 column(150 mm × 2.0 mm, 5 μm) by gradient elution using a mobile phase composed of water and acetonitrile adjusted to pH 3.0 at a flow rate of 0.25 mL/min, and multi-wavelength detection was used. The ion trap mass spectrometry was operated in the positive ion mode with scan range m/z 50 to 1 200 for qualitative and quantitative analysis of gallic acid and 11 other compounds. A good separation of 15 common peaks of Virgara Shiraz wine was achieved within 60 min, and 6 of them were confirmed by comparison with reference substances, and the similarity among 10 batches of Virgara Shiraz wines was all higher than 0.9. The developed fingerprint method is simple, sensitive, stable, accurate, and suitable for the quality evaluation of Virgara Shiraz wine.
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