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水稻种子耐贮藏性QTL主效应和上位性效应分析

作  者:
沈圣泉;庄杰云;王淑珍;杨国花;夏英武
单  位:
中国水稻研究所国家水稻改良中心;浙江省富阳市农业广播学校;浙江大学农业与生物技术学院;嘉兴市农业科学研究院
关键词:
水稻;RIL群体;QTL定位;主效应;上位性效应;耐贮藏性
摘  要:
利用珍汕97B/密阳46构建的RIL群体及其相应分子遗传图谱,采用种子加速老化鉴定法,以处理后的相对发芽率(%)作为该材料耐贮藏性的考察指标,分别对老化处理7d和14d后测得的数据进行QTL定位和上位性分析。共检测到2个主效应QTL与控制种子耐贮藏性有关。在老化程度相对较轻时,qSS9-1起忍耐作用;而在老化程度相对较重时,则有qSS4基因在起作用。试验还检测到5对耐贮藏性的上位性互作QTL,其贡献率为8.14% ̄13.51%,涉及第1、2、3、4、5、6、9、12等8条染色体。其中在7d老化处理试验中,检测到2对效应值相对较小的上位性互作;而在14d老化处理试验中,检测到3对效应值相对较大的上位性互作。
译  名:
Analysis of QTLs with Main and Epistatic Effects of the Seed Storability in Rice
作  者:
Shen Shengquan1* Zhuang Jieyun2 Wang Shuzhen3 Yang Guohua4 Xia Yingwu1 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 31002 9; 2 Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006; 3 Jiaxing Agricultural Science Research Academe, Ji axing, 314500; 4 Agricultural Broadcasting College of Fuyang in Zhejiang, Fuyang , 311400
关键词:
Rice, RIL population, QTLs mapping, Main effects, Epistatic effects, Storability
摘  要:
The recombinant inbred line (RIL) populations derived from Zhenshan 97B /Miyang 46 and its genetic linkage map were used to map the QTLs controlling the seed storability. This trait was measured by using the approaches to accelerate aging and indexed as relative germination rate (%). The trait was investigated after 7 days and 14 days of aging treatment. Two QTLs with main effects were det ected significantly for seed storability. When the slight aging treatment was ta ken, qSS9-1 played its roles in tolerance, but higher aging treatment was taken, qSS4 exerted its roles. Five pairs of epistasis QTLs was detected on chromosome s 1, 2, 3, 4, 5, 6, 9 and 12, each explained the total phenotypic variation of 8 .14%~13.51%. Of them, two epistasis were detected in the 7 days aging treatment and three were identified in 14 days of treatment.

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