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Position: Home > Articles > Identification of IgE-binding Epitopes on α_(s1)-Casein FOOD SCIENCE 2010,31 (17) 232-235

α_(s1)-酪蛋白IgE抗原决定簇的识别

作  者:
丛艳君;任发政;云战友
单  位:
北京工商大学化学与环境工程学院;中国农业大学食品科学与营养工程学院;内蒙古伊利实业集团股份有限公司
关键词:
牛奶;αs1-酪蛋白;IgE;抗原决定簇
摘  要:
以αs1-酪蛋白氨基酸序列为模板,错位合成αs1-酪蛋白多肽,以收集到的牛乳过敏患者血清为抗体,鉴定αs1-酪蛋白IgE抗原决定簇,分析影响致敏性的关键氨基酸,探讨牛乳过敏机理。结果表明:αs1-酪蛋白IgE抗原决定簇有5条,它们在αs1-酪蛋白的定位分别为aa21~35、aa26~40、aa91~105、aa141~155、aa186~200。影响αs1-酪蛋白致敏性的关键氨基酸为组氨酸、谷氨酸、脯氨酸。说明特异性水解抗原决定簇或定点修饰过敏原氨基酸实现脱敏的方法是可行的。
译  名:
Identification of IgE-binding Epitopes on α_(s1)-Casein
作  者:
CONG Yan-jun1,REN Fa-zheng2,*,YUN Zhan-you3 (1.School of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China ;2.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China ;3.Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010080,China)
关键词:
cow milk;αs1-casein;IgE;epitope
摘  要:
A series of casein overlapping decapeptides (offset by 5 amino acids) were synthesized on the basis of the sequence of αs1-casein.IgE-binding epitopes were identified using individual sera from cow milk allergic patients.Moreover,the critical amino acids (AAs) for IgE binding were explored.The results indicated that five IgE-binding epitopes were identified to locate in the sequence of aa21-35,aa26-40,aa91-105,aa141-155 and aa186-200,respectively.In addition,histidine,glutamic acid and proline were the critical amino acids for the allergenicity of αs1-casein.

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