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当前位置: 首页 > 文章 > 抗菌肽基因Gnk2-1经花粉管通道法导入西瓜的初步研究 园艺学报 2014,41 (7) 1467-1475
Position: Home > Articles > Introduction of Antimicrobial Peptide Gene Gnk2-1 into Watermelon via Pollen-tube Pathway Acta Horticulturae Sinica 2014,41 (7) 1467-1475

抗菌肽基因Gnk2-1经花粉管通道法导入西瓜的初步研究

作  者：
许珺然;张显;张勇;马建祥
单  位：
西北农林科技大学园艺学院
关键词：
西瓜;花粉管通道法;分子检测;抗菌肽
摘  要：
通过花粉管通道介导银杏抗菌肽基因Gnk2-1于西瓜自交系‘04-1-2’中,以浓度为100、200、300和400 ng·μL-1的DNA为供体,分别于授粉后24、27、30和33 h导入,对子代进行PCR检测。结果表明:T0代坐果率和单瓜结籽数随授粉后处理时间的增加而升高,但在各个处理时间下均显著低于对照;外源DNA的导入对授粉后27 h处理的T1代种子发芽率和授粉后24 h处理的T1代种子出苗率均造成显著降低;不同DNA浓度转化的T1代种子的发芽率和出苗率与对照相比差异不显著;对1 280株T1幼苗进行PCR检测,得到32株阳性转化植株,总体转化率为2.5%,最佳转化时间为授粉后24~27 h,最佳DNA转化浓度为100~200 ng·μL-1;PCR阳性植株抗病鉴定表明转基因植株对西瓜枯萎病的抗性有所增强。
译  名：
Introduction of Antimicrobial Peptide Gene Gnk2-1 into Watermelon via Pollen-tube Pathway
作  者：
XU Jun-ran;ZHANG Xian;ZHANG Yong;MA Jian-xiang;College of Horticulture,Northwest A&F University;
关键词：
watermelon;;pollen-tube pathway;;molecular detection;;antimicrobial peptide
摘  要：
In order to study the feasibility of transferring exogenous DNA into watermelon by pollen-tube pathway,the antimicrobial peptide gene Gnk2-1 was introduced into watermelon accession ‘04-1-2'by pollen-tube pathway with different concentration(100,200,300 and 400 ng · μL-1)at different time(24 h,27 h,30 h,33 h)after self-pollination. Seeds from treated fruits were harvested and tested by PCR. The results were as follows:The fruit setting rate and average number of seeds of T0 generation increased with the increment of treatment time after self-pollination,though the two figures both showed a significant decrease under all treatments compared with control. The germination rate of T1 seeds treated 27 h after self-pollination and the seedling emergence rate of T1 seeds treated 24 h after self-pollination decreased significantly. Compared with control,there was no distinct difference illustrated in the rate of germination and seedling emergence of T1 seeds between different concentration treatments. The PCR results of 1 280 tested T1 seedlings showed 32 positive plants and the total transformation rate was 2.5%. The optimum transformation time was 24–27 h after self-pollination and the optimum transformation concentration were 100–200 ng · μL-1. The positive plants were inoculated with Fusarium and showed a stronger resistance compared with non transgenic plants.

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