当前位置: 首页 > 文章 > 响应曲面法优化超声波提取红肉蜜柚红色素工艺 安徽农业科学 2012,40 (28) 13993-13996+14154
Position: Home > Articles > Optimization of the Ultrasonic Extraction of Red Pigments from Red-flesh Sweet Pomelo by Response Surface Methodology Journal of Anhui Agricultural Sciences 2012,40 (28) 13993-13996+14154

响应曲面法优化超声波提取红肉蜜柚红色素工艺

作  者:
胡位荣;林斌;李婉君;孙茹;刘顺枝
单  位:
广州大学生命科学学院
关键词:
红肉蜜柚;红色素;超声波提取;响应曲面法
摘  要:
[目的]寻找红肉蜜柚果实红色素的最佳提取方案。[方法]以红肉蜜柚果实为材料,在单因素试验的基础上,利用响应曲面法对果肉红色素超声提取工艺参数进行优化研究。选择超声浸提时间、浸提温度、料液比为自变量,以红色素提取液在471 nm处吸光度为响应值,采用Box-Behnken设计方法,模拟得出了红肉蜜柚红色素提取的回归方程。[结果]石油醚为提取剂时,超声辅助提取使红肉蜜柚果肉红色素吸光度增加106.1%;提取的最佳工艺条件为以石油醚为浸提溶剂,浸提温度22℃,浸提时间32 min,料液比1∶4.3 g/ml,此时红肉蜜柚果实红色素提取液的吸光度为1.312。[结论]研究可为红肉蜜柚红色素的开发利用提供参考依据。
译  名:
Optimization of the Ultrasonic Extraction of Red Pigments from Red-flesh Sweet Pomelo by Response Surface Methodology
作  者:
HU Wei-rong et al(School of Life Sciences,Guangzhou University,Guangzhou,Guangdong 510006)
关键词:
Red-flesh sweet pomelo;Red pigments;Ultrasonic-assisted extraction;Response surface methodology
摘  要:
[Objective] To find out the best extraction conditions of red pigments from red-flesh sweet pomelo.[Method] Based on single-factor test,the optimum ultrasonic extraction conditions of red pigments from red-flesh sweet pomelo were investigated by response-surface methodology.Three factors including extraction temperature,ultrasonic extraction time and material-liquid ratio were selected to maximize the absorbance of red pigments at 471 nm with Box-Behnken design and the regression equation was stimulated.[Result] The ultrasound-assisted extraction increased the absorbance of red pigments by 106.1%,with petroleum ether as extraction solvent,the optimum extraction parameters of red pigments were extraction temperature of 22 ℃,ultrasonic extraction time of 32 minutes and solid-liquid ratio of 1∶ 4.3(g/ml).Under the optimal extraction conditions,the absorbance of red pigments was 1.312.[Conclusion] The study provides references for the development and utilization of red pigments from red-flesh sweet pomelo.

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