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当前位置: 首页 > 文章 > 水稻种子贮藏10年后的活力相关基因定位 植物遗传资源学报 2015,16 (5) 1067-1072
Position: Home > Articles > Mapping of Quantitative Trait Loci Associated with Seed Longevity of Rice after Nearly 10 Years of Seed Storage Journal of Plant Genetic Resources 2015,16 (5) 1067-1072

水稻种子贮藏10年后的活力相关基因定位

作  者：
刘文强;黎用朝;李小湘;陆婷婷;潘孝武;盛新年;段永红;孙桂华
单  位：
湖南省水稻研究所
关键词：
水稻;数量性状座位;种子活力
摘  要：
以2004年构建并保存在种质库10年的186个单株组成的湘743/Katy F2:3群体为材料,在发芽的第5天和第9天统计亲本和各株系的发芽率和成苗率,应用由129个标记组成的连锁图谱检测与种子活力相关的QTL,一共检测到12个QTLs,共分布在6条染色体的6个区间,单个QTLs对群体性状表型变异的贡献率为5.73%~47.53%,联合贡献率都是50%。其中,在第8染色体RM152~RM310区间检测到一个主效的QTL,对第5天发芽率和第9天发芽率和第9天成苗率的贡献率分别为12.02%、47.53%、38.64%,来自于湘743的基因增加发芽率和成苗率;在第9染色体RM444~RM219区间检测到一个稳定表达的QTL,对第5天发芽率和第9天发芽率和第9天成苗率的贡献率分别为8.85%、7.49%、10.36%,来自于Katy的基因增加发芽率和成苗率;此外,没有检测到显著的上位性互作。
译  名：
Mapping of Quantitative Trait Loci Associated with Seed Longevity of Rice after Nearly 10 Years of Seed Storage
作  者：
LIU Wen-qiang;LI Yong-chao;LI Xiao-xiang;LU Ting-ting;PAN Xiao-wu;SHENG Xin-nian;DUAN Yong-hong;SUN Gui-hua;Hunan Rice Research Institute;
关键词：
rice(Oryza stativa L.);;quantitative trait locus(QTL);;seed longevity
摘  要：
In this paper,a F2: 3population of 186 linesstored in gene bank for ten years at Hunan Rice Research Institute( HNRRI) which were derived from Xiang 743 / Katy and was tested for germination percentage and normal seedling percentage in the fifth and ninth day at seed germination stage. A linkage map consisting of 129 SSR markers was employed to detect quantitative trait loci( QTL) associated with seed longevity. A total of 12 QTLs were detected,distributing on 6 regions in the genome,with phenotypic variance explanation anging as 5. 73% ~ 47. 53%and general contribution as high as 50%. Among them,a major QTL was identified in the interval of RM152-RM310 on chromosome 8,with germination percentage contribution of 12. 02% in the fifth day and germination percentage contribution of 47. 53% and normal seedling percentage contribution of 38. 64% in the ninth day,the allele for Xiang 743 played positive effect. Meanwhile,a stable QTL was detected in the interval of RM444- RM219 on chromosome 9,with germination percentage contribution of 8. 85% in the fifth day and germination percentage contribution of 7. 49% and normal seedling percentage contribution of 10. 36% in the ninth day,the allele from Katy played positive effect. In addition,no epistatic interaction was detected amongputative QTLs.

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