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Position: Home > Articles > Detection and Analysis of the Pig Sperm Quality by Flow Cytometry China Animal Husbandry & Veterinary Medicine 2016,43 (9) 2502-2507

猪精子质量的流式细胞检测分析

作  者:
吴梦;丁玉春;刘作华;刘文;罗林;葛良鹏
单  位:
重庆市畜牧科学院
关键词:
荣昌猪;精子;流式细胞术;检测
摘  要:
本试验将流式细胞术应用于猪精子分析,同时检测精子功能的多个特性,以保证精子具备多种特性且功能完整,顺利完成授精过程。分别采用SYBR-14/PI(Propidium Iodide)、YO-PRO-1/PI、PNA/PI及Mito Tracker/YO-PRO-1 4种染色方法对荣昌猪精子的质膜完整性、细胞凋亡、顶体完整性及线粒体功能进行了检测和分析;以SYBR-14~+/PI~-表示质膜完整的活精子,YO-PRO-1-/PI-表示活精子,PNA-/PI-表示未发生顶体反应的活精子,Mito Tracker~+/YO-PRO-1-表示线粒体活性高的活精子,以上述检测指标的综合结果判定精子功能的完整性。本试验通过流式细胞术对荣昌猪精子进行质量检测和分析,结果表明,该方法可为临床诊断种猪不孕症提供理论依据,对有效治疗不孕症有重要意义。
译  名:
Detection and Analysis of the Pig Sperm Quality by Flow Cytometry
作  者:
WU Meng;DING Yu-chun;LIU Zuo-hua;LIU Wen;LUO Lin;GE Liang-peng;Chongqing Academy of Animal Sciences;Key Laboratory of Pig Industry Sciences,Ministry of Agriculture;Scientific Observing and Experimental Station of Animal Housing System in Southwest Areas,Ministry of Agriculture;Chongqing Key Laboratory of Pig Industry Sciences;
关键词:
Rongchang pig;;sperm;;flow cytometry;;detection
摘  要:
The flow cytometry was used in this trail to determine the pig sperm quality,in order to ensure the sperm had complete functions during the fertilization process.Four stain methods of SYBR-14/PI(Propidium Iodide),YO-PRO-1/PI,PNA/PI,Mito tracker/YO-PRO-1were used to detect the plasma membrane integrity,apoptosis,top body ntegrity and mitochondrial function of Rongchang pig sperm.SYBR-14+/PI-expressed live sperm with complete plasma membrane,YO-PRO-1-/PI-expressed live sperm,PNA-/PI-expressed live sperm had no top body reaction,and Mito Tracker+/YO-PRO-1-expressed live sperm with high mitochondrial activity.The functional integrity of the tested sperm was evaluated according to the above comprehensive results.Through the study of Rongchang pig sperm quality detection by flow cytometry,it suggested that the method could provide theoretical basis for clinical diagnosis,and was very important for swine infertility treatments.

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