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Position: Home > Articles > Establishment of tissue culture and rapid propagation technology of Agave sisalana H.11648 Guangdong Agricultural Sciences 2011,38 (10) 26-28

剑麻H.11648组织培养快繁技术体系的建立

作  者:
黄麒参;陈玉生;洪向平;黄家总;钱兆军
单  位:
广东省农垦南亚热带作物科技中心
关键词:
H.11648麻;外植体;组织培养;快速繁殖
摘  要:
从外植体、基本培养基、激素水平及碳源的筛选方面进行H.11648麻组织培养快繁体系的建立研究。研究结果表明,珠芽苗茎尖为最理想的快速繁殖材料,芽诱导最佳培养基为MS+6-BA 5 mg/L+IBA 0.3 mg/L;继代增殖最适宜培养基为MMS+6-BA 5 mg/L+IBA 0.3 mg/L,增殖系数最高可达4.6;生根适宜培养基为1/2MMS+NAA 1.2 mg/L,生根率达到90%以上。将小苗移栽到农业土壤+泥碳土+沙(2∶1∶1)基质中,成活率达到95.5%。
译  名:
Establishment of tissue culture and rapid propagation technology of Agave sisalana H.11648
作  者:
HUANG Qi-shen,CHEN Yu-sheng,HONG Xiang-ping,HUANG Jia-zong,QIAN Zhao-jun (Guangdong Agribusiness South Aisa Tropical Crops Science and Technology Center,Guangzhou 510507,China)
关键词:
Agave sisalana H.11648;explants;tissue culture;rapid propagation
摘  要:
By screening aspects of the explants,the basic medium,hormone levels and carbon,this experiment established the tissue culture micropropagation system of Agave sisalana H.11648.The results showed that the optimal explants material for rapid propagation were bulbil shoot tip.The optimal shoot induction medium was MS+6-BA 5 mg/L+IBA 0.3 mg/L.The optimal subculture medium was MMS+6-BA 5 mg/L +IBA 0.3 mg/L with the multiplication factor of up to 4.6.The optimal rooting medium was 1/2MMS+NAA 1.2 mg/L,the rooting rate reached 90%.The rooted plantlets survied by 95.5% when transplanted onto containers for culture on the medium containing agricultural soil and peat and sand at ratio of 2∶1∶1.

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