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当前位置: 首页 > 文章 > 沙门菌感染雏鸡调节性T细胞变化的分析 畜牧兽医学报 2019,50 (11) 2302-2308
Position: Home > Articles > Analysis of Regulatory T Cells Changes in Chicks Infected with Salmonella Chinese Journal of Animal and Veterinary Sciences 2019,50 (11) 2302-2308

沙门菌感染雏鸡调节性T细胞变化的分析

作  者：
张锦;李庆贺;郑麦青;刘冉冉;崔焕先;文杰;赵桂苹
单  位：
中国农业科学院北京畜牧兽医研究所
关键词：
鸡;沙门菌;调节性T细胞;TGF-β
摘  要：
旨在分析雏鸡沙门菌感染后其脾调节性T细胞(Tregs)比例变化.本研究以京星黄鸡H系为试验群,将1日龄雏鸡随机分为对照组(n=40)和感染组(n=80),相同环境无菌饲养.7日龄时,感染组接种沙门菌建立感染模型,对照组以生理盐水代替沙门菌.感染48 h后,分别对两组雏鸡盲肠扁桃体免疫反应相关基因表达水平、血清炎症因子进行测定,并分别从中随机抽取雏鸡(对照组n=25,感染组n=34)检测其脾中CD4+T细胞、Tregs/CD4+T细胞比例.结果 显示:感染组与对照组相比,脾中CD4+T细胞比例无显著性差异(P>0.05).感染组与对照组CD4+ CD25+/CD4+(%)分别为0.93±0.12与3.22-±0.59(P<0.01);CD4+ TGF-β+/CD4+(%)分别为0.55±0.07与1.42±0.25(P<0.01);CD4+ CD25+ TGF-β+/CD4+(%)分别为0.29±0.04与0.76±0.14(P<0.01),均呈现显著性差异.雏鸡感染沙门菌后,脾中CD4+T细胞比例无显著性差异,而Tregs/CD4+T细胞比例较对照组明显下降(P<0.01).本研究提示调节性T细胞可能参与雏鸡沙门菌感染的发病过程,为进一步研究雏鸡抗沙门菌感染机制提供理论依据.
译  名：
Analysis of Regulatory T Cells Changes in Chicks Infected with Salmonella
作  者：
ZHANG Jin;LI Qinghe;ZHENG Maiqing;LIU Ranran;CUI Huanxian;WEN Jie;ZHAO Guiping;Institute of Animal Science, Chinese Academy of Agricultural Sciences,Beijing;
关键词：
chicks;;Salmonella;;Tregs;;TGF-β
摘  要：
The objective of this study was to analyze the proportion of spleen regulatory T cells(Tregs) in chicks after Salmonella infection. In this study, Jing xing yellow chicken H lines were used as experimental groups, and 1-day-old chicks were randomly divided into control group(n=40) and infected group(n=80).Animals were kept in the same and sterile environment. At 7 days of age, the infected group was infected with Salmonella(Salmonella typhimurium CICC 21484) to establish an infection model. The control group was treated by saline instead of Salmonella. After 48 hours of infection, the expression levels of cecal tonsil immune response-related genes and serum inflammatory factors were measured in two groups. Then, two groups of chicks were randomly selected(control group, n=25; infected group, n=34) to detect CD4~+ T cells and Tregs/CD4~+T cells ratio of spleens. The results showed that there was no significant difference in the proportion of CD4~+T cells in the spleens between control gruop and infected group(P>0.05). In infected group and control group, CD4~+ CD25~+/CD4~+T cells(%) were 0.93±0.12 and 3.22±0.59, respectively(P< 0.01); CD4~+ TGF-β~+/CD4~+T cells(%) were 0.55±0.07 and 1.42±0.25(P<0.01), respectively; CD4~+CD25~+ TGF-β~+/CD4~+T cells(%) were 0.29±0.04 and 0.76±0.14, respectively(P<0.01), there were significant differences between the above data of the two groups. There was no significant difference in the proportion of CD4~+T cells in the spleens of chicks after Salmonella infection. The ratio of Tregs/CD4~+T cells were significantly lower than that in the control group(P<0.01). It is suggested that Tregs may be involved in the pathogenesis of Salmonella infection in chicks, which provides a theoretical basis for further study on the mechanism of chicks against Salmonella infection.

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