作 者：
李岳诗;黎淳锋;张玉静;王艳路;郑霞林;陆温
摘 要：
【目的】对前人的眉斑并脊天牛(Glenea cantor Fabricius)幼虫饲养方法进行改进,以获得大量虫源,为系统研究该虫提供虫源保障。【方法】采用50 m L平底生物离心管,内置小木段,将眉斑并脊天牛幼虫转移至离心管内的小木段上,在温度(25±1)℃、相对湿度(75±5)%、光周期14L∶10D的条件下饲养,观察幼虫羽化情况,将羽化的成虫分别称重并统计性比、幼虫存活率和羽化率。【结果】所饲养的幼虫总存活率达(88.9±1.96)%,平均羽化率达(96.49±1.06)%,雌、雄成虫平均体重分别达0.2174±0.0011 g和0.1086±0.0052 g,成虫体重与自然种群无显著差异(P>0.05);幼虫正常发育对饲料量有一定要求,每头幼虫的饵料平均需要量为26.01~34.00 g,低于26.00 g的饲料量饲养出来的成虫体重明显偏小,与自然种群差异显著(P<0.05)。【结论】50 m L平底生物离心管法是一种一次性接虫、不需更换饲料、耗材少的饲养方法,可用于眉斑并脊天牛幼虫的定量饲养。
译 名：
Evaluation on effect of a new rearing method for Glenea cantor Fabricius larvae
作 者：
LI Yue-shi;LI Chun-feng;ZHANG Yu-jing;WANG Yan-lu;ZHENG Xia-lin;LU Wen;Agricultural College, Guangxi University;
关键词：
Glenea cantor Fabricius;;larvae;;rearing method;;rearing effect
摘 要：
【Objective】The present experiment was conducted to improve rearing method of G. cantor Fabricius larva to get a large number of G. cantor Fabricius, in order to provide guarantee for systematic research of G. cantor Fabricius.【Method】G. cantor Fabricius larva was transferred to short-cut wood within 50 m L centrifuge tube. Then the larva was incubated under temperature of( 25±1) ℃, photoperiod of L12∶D12 and relative humidity of( 75±5) %,meanwhile the larva eclosion was observed, and the adult's weight and sex ratio, and larva's pupation rate, survive rate and eclosion rate were determined and investigated. 【 Result 】 The results showed that the survival rate and eclosion rate of larvae were( 88.9±1.96) % and( 96.4±1.06) %, respectively. And the body weights of male and female adults were 0.2174±0.0011 g and 0.1086±0.0052 g, respectively, and had no significant difference from those of natural population(P>0.05). There are some requirements for normal development of larva, and the average feed requirement of every larva was 26.01-34.00 g. Thus the body weight of adult reared with less than 26.0 g was relatively lower, which had significant difference from that of natural population(P<0.05). 【Conclusion】The method for rearing G. cantor Fabricius in 50 m L centrifuge tube can simplify rearing procedures, such as inoculating larva once, not needing to renew feed, expending less materials.
