Position: Home > Articles > Studies on Multiplex RT-PCR Detection on Viruses of Lily
Hubei Agricultural Sciences
2013,52
(20)
5051-5053
百合病毒的多重RT-PCR检测技术
作 者：
邹迎春;杨朝柱;覃大吉;赵清华;向极钎;殷红清;杨永康;黄建民;皮磊
单 位：
恩施清江生物工程有限公司;湖北省农业科技创新中心鄂西综合试验站
关键词：
百合(Lilium brownii var.viridulum);病毒;多重RT-PCR
摘 要：
根据百合(Lilium brownii var.viridulum)常见的黄瓜花叶病毒(CMV)、百合斑驳病毒(LMoV)、无症病毒(LSV)引物序列,设计2组特异引物,以18S rRNA为内参基因,采用多重RT-PCR,2组引物均能扩增得到LMoV和LSV预期大小一致的片段,未检测到CMV,检测结果不受病株取材部位的影响。该方法的建立为百合脱毒苗的规模化生产提供了高效检测技术。
译 名：
Studies on Multiplex RT-PCR Detection on Viruses of Lily
作 者：
ZOU Ying-chun;YANG Chao-zhu;QIN Da-ji;ZHAO Qing-hua;XIANG Ji-qian;YIN Hong-qing;YANG Yong-kang;HUANG Jian-min;PI Lei;Enshi Qingjiang Bio-Engineering Co., Ltd.;West Comprehensive Experiment Station of Hubei Provincial Agriculture S&T Innovation Center;
关键词：
lily(Lilium brownii var.viridulum);;virus;;multiplex RT-PCR
摘 要：
Based on the primers detecting lily(Lilium brownii var. viridulum) cucumber mosaic virus(CMV), lily mottle virus(LMoV) and lily symptomless virus(LSV), two groups of specific primers were designed. Using 18S ribosomal RNA as internal control gene, multiplex RT-PCR method were established to detect the 3 major viruses. LMoV and LSV were detected, but CMV was not detected. This detecting method will promote the large-scale production of the virus-free lily seedling.
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