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Position: Home > Articles > Chlorophyll a / b Binding Proteins Thcab1 Gene Clone and Expression Analysis of Tamarix hispida Journal of Northeast Forestry University 2014 (2) 54-58

刚毛柽柳光捕获叶绿素a/b结合蛋白基因Thcab1的克隆与分析

作  者:
刘亚丽;张凯敏;高彩球
单  位:
林木遗传育种国家重点实验室(东北林业大学);东北林业大学
关键词:
刚毛柽柳;叶绿素a/b结合蛋白基因;盐胁迫
摘  要:
通过对NaHCO3胁迫后刚毛柽柳(Tamarix hispida)7个转录组分析,鉴定获得了一个叶绿素a/b结合蛋白基因(命名为Thcab1)全长cDNA序列,该基因全长1 293 bp,编码区长822 bp,编码含273个氨基酸的蛋白质,编码蛋白的分子量为29.44 ku,理论等电点为7.84,NaHCO3胁迫不同时间点转录组数据表明,Thcab1基因的表达主要表现为受盐碱胁迫抑制。为了研究该基因对NaCl胁迫不同时间的应答情况,采用实时定量RT-PCR技术分析刚毛柽柳在NaCl胁迫处理后不同时间、不同组织中基因的表达。结果表明,在刚毛柽柳根和叶中,NaCl胁迫24 h内Thcab1基因主要表现为上调表达。
译  名:
Chlorophyll a / b Binding Proteins Thcab1 Gene Clone and Expression Analysis of Tamarix hispida
作  者:
Liu Yali;Zhang Kaimin;Gao Caiqiu;Northeast Forestry University;State Key Laboratory of Forest Genetics and Tree Breeding,Northeast Forestry University;
关键词:
Tamarix hispida;;Chlorophyll a/b binding proteins;;Salt stress
摘  要:
The full length cDNA of a light-harvesting chlorophyll a/b-binding protein( named Thcab1) gene was isolated from seven transcriptome cDNA librarys of Tamarix hispida under NaHCO3stress. The Thcab1 cDNA was 1 293 bp,and open reading frame( ORF) was 822 bp,encoding a protein of 273 amino acid residues with the molecular mass was 29. 44 ku and theoretical pI was 7. 84. The results of transcriptomes show that Thcab1 is obvious down-regulated under NaHCO3stress. The expressions of Thcab1 in T. hispida responding to NaCl in different tissues at diffident period are further investigated by using quantitative RT-PCR. Thcab1 is induced in roots and leaves of T. hispida during 24 hours under NaCl stress.

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