Position: Home > Articles > Determination of Fructooligosaccharides in Milk and Milk Products by High Performance Ion Exchange Chromatography
Journal of Dairy Science and Technology
2012,35
(1)
40-42
高效离子色谱法测定乳及乳制品中低聚果糖含量
作 者：
刘玉峰;李东;唐华澄;张绩觅
单 位：
北京市营养源研究所分析检测中心
关键词：
高效离子色谱法;脉冲安培检测;低聚果糖
摘 要：
建立离子色谱法定量测定乳及乳制品中低聚果糖的方法,应用CarboPacTMPA1(4mm×250mm)阴离子交换柱、氢氧化钠和醋酸钠淋洗液梯度淋洗、脉冲安培检测器检测。6次测定液态奶中低聚果糖的含量,相对标准偏差小于9%,3种糖的加标回收率在94.8%～103.6%之间。本方法简单快捷,适用于乳及乳制品中低聚果糖含量的检测。
译 名：
Determination of Fructooligosaccharides in Milk and Milk Products by High Performance Ion Exchange Chromatography
作 者：
LIU Yu-feng,LI Dong,TANG Hua-cheng,ZHANG Ji-mi(Analysis Laboratory of Beijing Nutrition Resources Institute,Beijing 100069,China)
关键词：
high performance ion exchange chromatography(HPIEC);pulsed amperometric detection(PAD);fructooligosaccharides(FOS)
摘 要：
An analytical method was established to determine fructooligosaccharides(FOS) in milk and milk products by high performance ion exchange chromatography method with pulsed amperometric detection.The column was a CarboPac PA1(4 mm × 250 mm) anion exchange column.Fructooligosaccharides were separated under gradient elution mode.The mobile phase A was 150 mmol/L NaOH and the mobile phased B was composed of 150 mmol/L NaOH and 100 mmol/L sodium acetate.The relative standard derivations for 6 replicate determinations of four fructooligosaccharides in liquid milk were less than 9%.The average spike recovery rates for three fructooligosaccharides in liquid milk varied between 94.8% and 103.6%.The method was simple,fast and suitable for determining FOS in milk and milk products.
导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
