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Position: Home > Articles > Construction of cold-inducible normalized full-length cDNA library of Ceratoides lanata Journal of Central South University of Forestry & Technology 2011,31 (7) 169-173

绵毛优若藜冷胁迫均一化全长cDNA文库的构建

作者：

明付焕;张党权;宋志丹;谷振军;祝全东;韩欣;郭林林

单位：

中南林业科技大学国家林业局栽培与育种重点实验室

关键词：

绵毛优若藜;全长cDNA文库;均一化;冷胁迫;抗逆基因

摘要：

以绵毛优若藜叶片和嫰茎为材料,对盆栽的绵毛优若藜小苗进行梯度低温胁迫处理后,采用SMART(switching mechanism at 5′end of RNA transcript)与DSN(duplex-specific nuclease)均一化相结合技术构建绵毛优若藜冷胁迫均一化全长cDNA文库。经测定,原始文库的库容量为1.4×106pfu。PCR检测结果显示:插入片段的长度在0.5~3 kb之间,平均大于1 kb,表明文库构建效果较好。该文库包含有大量的未知基因,有待进一步的发掘和研究。

译名：

Construction of cold-inducible normalized full-length cDNA library of Ceratoides lanata

作者：

MING Fu-huan,ZHANG Dang-quan,SONG Zhi-dan,GU Zhen-jun,ZHU Quan-dong,HAN Xin,GUO Lin-lin(The Key Lab of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

关键词：

Ceratoides lanata;full-length cDNA library;normalization;cold-stress;stress-resistant genes

摘要：

Taking the leaves and young stems as material,a normalized full-length cDNA library of Ceratoides lanata was constructed.The seedlings of potted Ceratoides lanata were treated by gradient cold stress,normalized full-length cDNA library was successfully constructed by DSN(duplex-specific nulease)-normalization combined with SMARTTM(switching mechanism at 5′end of RNA transcript) technique.The results show that the capacity of the primary library was 1.4×106 pfu.The PCR results show that the insertion size was between 0.5 kb and 3.0kb,averagely about 1.0kb,suggesting that the cDNA library was well normalized and had been established successfully.The analytic result suggested that this cDNA library contains large new genes,which need further researches and functional analysis.

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绵毛优若藜冷胁迫均一化全长cDNA文库的构建

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中南林业科技大学学报

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