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Position: Home > Articles > Cloning and Sequencing Analysis of the Major Surface Protein P32 Gene of Theileria luwenshuni Progress in Veterinary Medicine 2010,31 (2) 11-14

吕氏泰勒虫主要表面蛋白P32基因的克隆与序列分析

作  者:
刘爱红;关贵全;刘军龙;李有全;马米玲;牛庆丽;任巧云;殷宏;罗建勋
单  位:
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室农业部草食动物疫病重点实验室
关键词:
吕氏泰勒虫;表面蛋白P32基因;克隆;序列分析
摘  要:
从吕氏泰勒虫宁县株中提取RNA,用RT-PCR方法扩增主要表面蛋白P32基因的cDNA片段,克隆后测定其核苷酸序列,并推导了相应的氨基酸序列。结果表明,扩增的P32基因长度为875个核苷酸,共编码284个氨基酸。核苷酸序列与已发表的牛的4种泰勒虫的主要表面蛋白基因相比较,其与瑟氏泰勒虫俄罗斯株、日本株、中国辽阳株的核苷酸和氨基酸序列相似性均在98.9%以上。其推导的氨基酸序列中有3个糖基化位点。
译  名:
Cloning and Sequencing Analysis of the Major Surface Protein P32 Gene of Theileria luwenshuni
作  者:
LIU Ai-hong,GUAN Gui-quan,LIU Jun-long,LI You-quan,MA Mi-ling,NIU Qing-li,REN Qiao-yun,YIN Hong,LUO Jian-xun(Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory on Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China)
关键词:
Theileria luwenshuni;surface protein P32 gene;clone;sequence analysis
摘  要:
The major surface protein P32 gene of Theileria luwenshuni isolated from Ningxian county in Gansu province was successfully amplified by RT-PCR. The amplicon was 875bp in length,coding for 284 amino acid residues,and had above 98.9% of identity with three strains T.sergenti from Jap/Rus/ China.The deduced peptide of 284 amino acid residues contained 3 glycosylation sites. The research data were submitted to GenBank and gained a new accession No.GQ281044.The result provided the foundation of the immunological diagnosis and prevention for ovine theileriosis in our county.

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