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Position: Home > Articles > Genotypic Detection and Drug Resistance Analysis of Escherichia coli ESBLs in Henan Province Journal of Shanxi Agricultural Sciences 2018 (12) 2087-2093

河南省猪源大肠杆菌ESBLs基因型检测及耐药性分析

作  者:
张青娴;徐引弟;王治方;朱文豪;焦文强;李海利;方剑玉;郎利敏;张立宪;游一;许峰;郑万录;王克领
单  位:
河南省农业科学院畜牧兽医研究所;河南省畜禽繁育与营养调控重点实验室
关键词:
大肠杆菌;ESBLs;基因型;耐药
摘  要:
对河南省农业科学院畜牧兽医研究所传染病研究室2018年1—4月所分离的70株河南省猪源大肠杆菌,用双纸片法初步确证30株疑似ESBLs菌株,阳性率达42.8%。对30株疑似ESBLs菌株扩增其耐药基因,其阳性率分别为CTX-M53.3%,TEM66.7%,SHV 3.3%,未扩增出OXA,DHA基因。结果显示,河南省农业科学院畜牧兽医研究所传染病研究室所分离的河南省猪源ESBLs大肠杆菌耐药基因型以CTX-M和TEM型为主,且多为耐药菌株。
译  名:
Genotypic Detection and Drug Resistance Analysis of Escherichia coli ESBLs in Henan Province
作  者:
ZHANG Qingxian;XU Yindi;WANG Zhifang;ZHU Wenhao;JIAO Wenqiang;LI Haili;FANG Jianyu;LANG Limin;ZHANG Lixian;YOU Yi;XU Feng;ZHENG Wanlu;WANG Keling;Institute of Animal Husbandry and Veterinary,Henan Academy of Agricultural Sciences;Key Laboratory of Livestock Breeding and Nutrition Regulation,Henan Province;
关键词:
Escherichia coli;;ESBLs;;genotype;;drug resistance
摘  要:
70 strains of Henan pig-derived Escherichia coli isolated from January to April 2018 in the Infectious Diseases Laboratory of Institute of Animal Husbandry and Veterinary, Henan Academy of Agricultural Sciences, 30 strains of suspected ESBLs strains were initially confirmed by the double-disk method. The positive resistance rates of 30 suspected ESBLs strains were 53.3% for CTX-M, 66.7% for TEM, and 3.3% for SHV. OXA and DHA gene were not amplified. The results showed that the resistant genotypes of ESBLs-producing Escherichia coli isolates from Henan province were mainly CTX-M and TEM type, and most of them were drug-resistant strains.

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