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当前位置: 首页 > 文章 > 嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响 食品科学 2012,33 (15) 279-282
Position: Home > Articles > Effect of Lactobacillus acidophilus on β-Lactoglobulin Induced Mouse Th1/Th2 Cell Imbalance FOOD SCIENCE 2012,33 (15) 279-282

嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响

作  者：
李艾黎;孟祥晨;徐渐;邵红;马冬雪
单  位：
乳品科学教育部重点实验室东北农业大学食品学院;东北农业大学食品学院
关键词：
嗜酸乳杆菌;β-乳球蛋白;牛乳过敏
摘  要：
目的:观察嗜酸乳杆菌对牛乳β-乳球蛋白(BLG)致敏小鼠Th1/Th2细胞平衡及血清抗体水平的影响,以研究其缓解过敏反应的作用。方法:用牛乳BLG和弗氏佐剂的混合液腹腔注射诱发BALB/c小鼠致敏,建立动物过敏模型。将实验动物随机分为空白组、过敏组与不同剂量的嗜酸乳杆菌组。采用ELISA法测定各组小鼠血清总IgE、BLG特异性IgE和总IgG含量。体外分离培养各组小鼠脾淋巴细胞,采用ELISA法检测细胞上清液中Th1型细胞因子(IL-12、IFN-γ)和Th2型细胞因子(IL-4)的水平。结果:中、高剂量嗜酸乳杆菌组小鼠的IFN-γ/IL-4比值(代表Th1/Th2细胞平衡)显著高于过敏组(P<0.05);而其血清中的总IgE、BLG特异性IgE和总IgG水平显著低于过敏组(P<0.05),与空白组相比无差异性(P>0.05)。结论:嗜酸乳杆菌干预可改善小鼠的BLG过敏症状,其作用可能与促进Th1占优势的Th1/Th2细胞平衡,阻断IgE及IgG分泌相关。
译  名：
Effect of Lactobacillus acidophilus on β-Lactoglobulin Induced Mouse Th1/Th2 Cell Imbalance
作  者：
LI Ai-li 1,MENG Xiang-chen 1,,XU Jian 2,SHAO Hong 1,MA Dong-xue 1(1.Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China;2.College of Food Science,Northeast Agricultural University,Harbin 150030,China)
关键词：
Lactobacillus acidophilus;β-lactoglobulin;milk allergy
摘  要：
Objective: To investigate the effects of Lactobacillus acidophilus on the Th1/Th2 cell balance and antibody production in bovine β-lactoglobulin(BLG)-sensitized mice and evaluate its efficacy to relieve consequent allergic reactions.Methods: BALB/c mice were intraperitoneally injected with a mixture of milk BLG and Freund,s adjuvant to create BLG-sensitized mouse models.The mice were randomly divided into blank group,allergic group and different dose of Lactobacillus acidophilus group.The total IgE,BLG-specific IgE and total IgG contents in the serum of mice were measured by ELISA.Splenic lymphocytes were isolated and cultured in vitro to detect the levels of Thl type cytokine(IL-12,IFN-γ) and Th2 type cytokine(IL-4) in the supernatant by ELISA.Results: The IFN-γ/IL-4 ratio,which represents the Th1/Th2 cell balance,increased significantly in both middle-and high-dose Lactobacillus acidophilus groups compared to the allergic group(P<0.05).At the same time,their serum levels of total BLG-IgE and total IgG decreased significantly(P < 0.05),but showed no significant difference compared to the blank control(P>0.05).Conclusion: Intragastric administration of Lactobacillus acidophilus may be effective in preventing and alleviating BLG allergic symptoms by restoring the Th1/Th2 cell balance to Th1 dominance and by inhibiting IgE and IgG production.

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