作 者：
王雪丽;路子云;李建成;李贞;董杰;刘小侠
单 位：
中国农业大学植物保护学院昆虫学系和农业部有害生物监测与绿色防控重点实验室;河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心农业部华北北部作物有害生物综合治理重点实验室;北京市植物保护站
关键词：
粘虫;Cry1Ac蛋白;Cry2Ab蛋白;杀虫活性;生长发育
摘 要：
【目的】为探究Bt杀虫蛋白对次要靶标害虫粘虫Mythimna separata (Walker)(鳞翅目:夜蛾科)的杀虫活性及对其生长发育的影响。【方法】本文通过浸叶法饲喂初孵及2龄末粘虫不同剂量的Cry1Ac及Cry2Ab杀虫蛋白后,观察其死亡率,称量幼虫重,并统计了幼虫历期、化蛹率、蛹重、蛹期、蛹的羽化率、畸形率等指标。【结果】初孵幼虫取食浸泡含16、64、128μg/mLCry1Ac及Cry2Ab的玉米叶片后,随着时间的延长及浓度的增加,死亡率逐渐增加,且Cry1Ac杀虫蛋白对粘虫的生物活性高于Cry2Ab蛋白,在128μg/mL浓度下,取食Cry1Ac和Cry2Ab蛋白13d时的死亡率分别达到了65%及60%。取食两种蛋白后,初孵幼虫和2龄末幼虫重量均受到显著抑制,短期取食两种蛋白对幼虫历期、化蛹率、蛹重、蛹期、蛹的羽化率、畸形率没有影响。【结论】取食Cry1Ac和Cry2Ab杀虫蛋白后,对初孵幼虫有很好的杀虫活性,且Cry1Ac杀虫活性高于Cry2Ab杀虫蛋白;短期饲喂两种杀虫蛋白时,对2龄粘虫后期生长影响不大。本文结果为转Bt基因作物更好的应用于粘虫的防治提供了理论基础。
译 名：
Effects of Cry1Ac and Cry2Ab protein on development of oriental armyworm, Mythimna separata(Walker)(Lepidoptera:Noctuidae)
作 者：
WANG Xue-Li;LU Zi-Yun;LI Jiang-Cheng;LI Zhen;DONG Jie;LIU Xiao-Xia;Department of Entomology and MOA Key Laboratory of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University;Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences; IPM Center of Hebei Province;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture;Beijing Plant Protect Station;
单 位：
WANG Xue-Li%LU Zi-Yun%LI Jiang-Cheng%LI Zhen%DONG Jie%LIU Xiao-Xia%Department of Entomology and MOA Key Laboratory of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University%Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences%IPM Center of Hebei Province%Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture%Beijing Plant Protect Station
关键词：
Mythimna separata;;CrylAc toxin;;Cry2Ab toxin;;insecticidal activity;;growth and development
摘 要：
[Objectives] To explore the insecticidal activity of Bt on the oriental armyworm, Mythimna separata(Walker)(Lepidoptera: Noctuidae), including its effects on larval growth and development. [Methods] The leaf-dip bioassay method was used to feed neonates and second instar armyworm corn leaves treated with different doses of Cry1 Ac and Cry2Ab insecticidal proteins, after which, mortality, larval weight, duration of the larval period, pupal weight, duration of the pupal period, pupation rate, emergence rate and abnormality rate, were recorded. [Results] The mortality of neonates fed leaves that had been immersed in 16, 64, 128 μg/mL Cry1 Ac and Cry2Ab protein, increased gradually with increasing insecticide concentration and feeding time. The biological activity of Cry1 Ac on larvae was higher than that of Cry2 Ab. At concentrations of 128 μg/mL Cry1 Ac and Cry2 Ab, mortality at 13 d reached 65% and 60%, respectively. Larval weights of neonates and second instars were both significantly inhibited. Short-term exposure had no effect on larval duration, pupal weight, pupal duration, pupation rate, emergence rate and abnormality rate. [Conclusion] Cry1 Ac and Cry2Ab proteins were highly toxic to newly hatched M. separata larvae, but the insecticidal activity of Cry1 Ac was higher than that of Cry2 Ab. Short-term exposure to either Bt toxin had little effect on the growth and development of second instar armyworms. These results provide a theoretical basis for selecting transgenic Bt crops to control armyworms.
