当前位置: 首页 > 文章 > 脂肽类生物表面活性剂微生物合成菌的筛选 湖北农业科学 2009,48 (11) 2719-2721
Position: Home > Articles > Isolation and Characterization of Microbes Producing Lipopeptide Surfactant Hubei Agricultural Sciences 2009,48 (11) 2719-2721

脂肽类生物表面活性剂微生物合成菌的筛选

作  者:
胡申才;周焱富;张宝
单  位:
武汉工业学院生物与制药工程学院
关键词:
生物表面活性剂;微生物筛选;脂肽
摘  要:
对采集的16个样品经富集培养和平板分离,共获得122株菌株。采用排油活性法和表面张力测定法筛选出5株产表面活性剂的优良菌株,均能将发酵液表面张力降低到40.0 mN/m以下,其中一株假单胞菌H0591菌株将发酵液表面张力下降到了32.2 mN/m。该菌产生的表面活性剂具有良好的酸碱稳定性和耐温性,且显示出了很强的抗真菌活性。薄层色谱(TLC)后通过茚三酮显色分析表明该表面活性剂为脂肽类化合物。
译  名:
Isolation and Characterization of Microbes Producing Lipopeptide Surfactant
作  者:
HU Shen-cai,ZHOU Yan-fu,ZHANG Bao(School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China)
关键词:
biosurfactant;microbial synthesis;lipopeptide
摘  要:
122 microbial cultures were isolated from 16 samples by the methods of enrichment culture and dilution spread.And 5 isolates capable of producing biosurfactant,which could decrease the surface tension to lower than 40.0 mN/m,were screened by hydrolyzing oil activity assay on oil agar plates and oil displacement activity.Among them,strain H0591,belonging to Pseudomonas,even could decrease the surface tension to 32.2 mN/m.The biosurfactant produced by the strain H0591 exhibited excellent stability against the pH and temperature,and also performed strong anti-fungi activity.The result of TLC and ninhydrin colorization showed that the surfactant was lipopeptide.

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊