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当前位置: 首页 > 文章 > 功能性饮料中9种水溶性维生素的HPLC-MS-MS同步检测技术 食品科学 2014,35 (12) 196-199
Position: Home > Articles > Simultaneous Determination of 9 Water Soluble Vitamins in Drinks by Liquid Chromatography Tandem Mass Spectrometry FOOD SCIENCE 2014,35 (12) 196-199

功能性饮料中9种水溶性维生素的HPLC-MS-MS同步检测技术

作  者：
夏静;俞婧;孙磊;张岩
单  位：
河北省食品质量监督检验研究院
关键词：
水溶性维生素;高效液相色谱-质谱;饮料
摘  要：
建立高效液相色谱-质谱法测定功能性饮料中9种水溶性维生素的定性定量同步检测方法。样品利用Supelco C18(500 mg)固相萃取柱萃取后,采用Agilent plus C18(100 mm×2.1mm,3.5μm)色谱柱进行分离;流动相为0.1%甲酸溶液和乙腈,梯度洗脱,采用电喷雾离子源多反应监测模式进行检测。结果表明,9种水溶性维生素在10~1 000 ng/mL范围内线性关系良好(R2≥0.997),检出限在0.157~0.836μg/L之间。在加标量为0.05、0.1 mg/L和1 mg/L水平下,待测物的平均回收率在80.11%~115.56%之间,测定结果的相对标准偏差小于10%。
译  名：
Simultaneous Determination of 9 Water Soluble Vitamins in Drinks by Liquid Chromatography Tandem Mass Spectrometry
作  者：
XIA Jing;YU Jing;SUN Lei;ZHANG Yan;Hebei Institute of Food Quality Supervision Inspection and Research;
关键词：
water soluble vitamin;;high performance liquid chromatography tandem mass spectrometry(HPLC-MS-MS);;drinks
摘  要：
A method was established for the simultaneous determination of 9 water soluble vitamins in drinks using high performance liquid chromatography tandem mass spectrometry interfaced with electrospray(HPLC-MS-MS). The pretreatment procedure using solid phase extraction with Supelco C18(500 mg) was simple. An Agilent plus C18(2.1 mm ×100 mm, 3.5 μm) column was used for the chromatographic separation, and gradient elution was performed with 0.1% formic acid and acetonitrile as the mobile phase. Mass spectral acquisition was done in the positive ion mode by applying multiple reaction monitoring. The method was validated in terms of linearity, sensitivity, repeatability, recovery and selectivity. Satisfactory results in the majority of the cases were obtained, with practical limits of detection acceptable for routine monitoring purposes. Average recoveries for the analytes ranged from 80.11% to 115.56% at spiking levels of 0.05, 0.1 and 1 mg/L. The intra- and inter-day precision(RSD) were both less than 10%.

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