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当前位置: 首页 > 文章 > 贵州黑山羊独立生长因子1B基因cDNA克隆与生物信息学分析 畜牧与兽医 2012,44 (7) 27-32
Position: Home > Articles > Cloning and bioinformatics analysis of GFI1B gene cDNA in the Guizhou Black Goat Animal Husbandry & Veterinary Medicine 2012,44 (7) 27-32

贵州黑山羊独立生长因子1B基因cDNA克隆与生物信息学分析

作  者：
陈志;罗卫星;刘若余;石照应;张依裕;蔡惠芬;谈为忠
单  位：
苏州市吴中区金庭动物防疫站;贵州大学动物科学学院
关键词：
贵州黑山羊;GFI1B;克隆;序列分析
摘  要：
选择与羊同源性较高的牛独立生长因子1B(GFI1B)基因序列设计特异性引物,提取贵州黑山羊脾脏总RNA,通过RT-PCR技术对GFI1B基因进行克隆测序及序列分析。结果表明:首次克隆了贵州黑山羊GFI1B基因cDNA序列996 bp,GenBank登录号为JN662390,编码331个氨基酸。贵州黑山羊GFI1B基因与牛的同源性高达97.0%。聚类分析显示:哺乳动物、禽类和两栖类各为一类。生物信息学分析表明:山羊GFI1B分子包括1个低组分复杂性区域、6个锌指结构域ZnF-C4。同时,预测结果还表明GFI1B分子不包含信号肽序列且没有跨膜螺旋结构域,26个磷酸化位点,蛋白糖基化位点有7个。
译  名：
Cloning and bioinformatics analysis of GFI1B gene cDNA in the Guizhou Black Goat
作  者：
CHEN Zhi1,LUO Wei-xing1*,LIU Ruo-yu1,SHI Zhao-ying1,ZHANG Yi-yu 1,CAI Hui-fen1,TAN Wei-zhong2(1.Ministry of Education Key Laboratory for Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,College of Animal Science,Guizhou University,Guiyang 550025,China; 2.Animal Epidemic Prevention Station of Suzhou Wuzhong District,Suzhou 215111,China)
关键词：
Guizhou Black Goat;Growth Factor Independent 1B;Cloning;Bioinformatics.
摘  要：
Cow GFI1B genome sequence highly homologinized with that of sheep was selected for design a specific primer.Total RNA was extracted from the Guizhou Black Goat of spleen and the cDNA encoding GFI1B was obtained by the reverse transcription PCR(RT-PCR).The purified RT-PCR product was cloned into T vector and the sequence was analyzed.The results demonstrated that the 996bp product was found in the Guizhou Black Goat GFI1B cDNA,and GenBank accession number were JN662390,respectively.It encoded 331 amino acids.GFI1B protein in the Guizhou Black Goat had 97.0% homology with cow.Cluster analysis revealed that probably mammals,birds and amphibians,each of these belonged to different categories,separately.Bioinformatics analysis indicated that goat GFI1B protein contained one low compositional complexity region,six ZnF-C4 without signal peptide,transmembrane helix,26 phosphorylation sites and 7 prodiction O-glycosylation sites.

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