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Position: Home > Articles > Induced expression of CHS gene in muskmelon fruit treated with sodium silicate and challenged with Trichothecium roseum Journal of Gansu Agricultural University 2010,45 (3) 53-56

采后硅酸钠处理及损伤接种对厚皮甜瓜CHS基因的诱导表达

作  者:
高雄杰;赵继荣;毕阳;李科玮;李红霞;伍利芬
单  位:
甘肃农业大学食品科学与工程学院;甘肃农业大学农学院
关键词:
厚皮甜瓜;硅酸钠;CHS基因;诱导表达
摘  要:
以厚皮甜瓜品种"银帝"为试材,根据黄瓜查儿酮合成酶(CHS)基因设计引物,运用半定量RT-PCR技术,研究了采后100mmol·L-1硅酸钠浸泡处理对甜瓜果实CHS基因表达的影响.结果表明:PCR扩增所得片段(494bp)与黄瓜CHS基因同源,相似性为94%,可用于半定量RT-PCR分析;CHS基因在处理和对照中的表达丰度极低,无法检测;损伤接种T.roseum后第4d,CHS基因大量表达,且硅酸钠处理+损伤接种与对照+损伤接种差异达到最大,前者高出后者12%.
译  名:
Induced expression of CHS gene in muskmelon fruit treated with sodium silicate and challenged with Trichothecium roseum
作  者:
GAO Xiong-jie1,2,ZHAO Ji-rong1,2,BI Yang1,LI Ke-wei1,LI Hong-xia1,WU Li-fen1(1.College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Key Lab of Crop Improvement&Germplasm Enhancement, Lanzhou 730070, China)
关键词:
muskmelon fruit;sodium silicate;chalcone synthase gene (CHS);induced expression
摘  要:
The primer used in this experiment was designed according to the CHS gene from cucumber.Semi quantitative RT-PCR was used for evaluating the effect of sodium silicate dipping at 100 mmol·L-1 on CHS gene expression of muskmelon fruit (Cucumis melon L. cv. Yindi). The results showed that the PCR segments(494 bp)which shared 94 % homology with the cucumber CHS gene could be used for RT-PCR;the expression of CHS gene in the treated fruit and the control were too low to detect.However,an abundance expression of CHS gene was found 4 days after challenged with T.roseum,and 12 % higher in the treated fruit + challenged than control + challenged.

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