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Journal of Shenyang Agricultural University
1995
(3)
马铃薯Y病毒复制酶基因植物表达载体构建及转基因烟草的培育
作 者：
王振东;刘玉乐;房德纯;吴元华;田波
单 位：
沈阳农业大学植保系;沈阳农业大学植保系，中国科学院微生物研究所
关键词：
马铃薯Y病毒，脉坏死株系，复制酶基因，植物表达载体，转基因烟草
摘 要：
用ＢａｍＨＩ／ＫｐｎＩ从重组克隆ｐＺＤ－１中切下马铃薯Ｙ病毒脉坏死株系复制酶基因，将其插入质粒ｐＲＯＫ２相应切点中构成植物表达载体．用重组ｐＲＯＫ２转化农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍｅｆａｃｉｅｎｓ）ＬＢＡ４４０４菌株，通过重组农杆菌侵染叶盘将复制酶基因转入烟草品种ＮＣ８９中获得转基因植株．分子生物学检测结果表明，复制酶基因在转基因烟草中获得表达，并转基因烟草表现一定程度的延迟发病作用．
译 名：
Construction of Plant Expression Vector of Potato Virus Y(N strain) and Culture of Transgenic Tobacco
作 者：
Wang Zhendong; Liu Yule; Fang Dechun; Wu Yuanhua;Tian Bo (Department of the Plant Protection,Shenyang Agricultural University,Ahenyang110161)(Institute of Microbiology,Academia Sinica,Beijing 100080)
关键词：
potato virus Y; N strain;replicase gene; plant expression vector;transgenic tobacco
摘 要：
In order to construction of the gene expression vector ,the NIb fragment were cloned into plant expression vector p Rok2 and recombinanted pROK2 was mated into Agrobacterium tumefaciens Conn 'LBA4404' by a direct transfere method and transformed into commecial tobacco cultivar NC89. The tobacco plants transformed with NIb gene were detected by northern blot analysis.The results showed that had transcripts in transgenic tobacco plants. The transgenic tobacco have some resistance to challenge PVYN.
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