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Position: Home > Articles > Effect of Baicalin and Forsythiaside on the Excretion of IFN-α and IFN-γ in Pulmonary Microvascular Endothelial Cells China Animal Husbandry & Veterinary Medicine 2009,36 (6) 30-34

黄芩苷、连翘酯苷对肺微血管内皮细胞分泌IFN-α、IFN-γ的影响

作  者:
林红;胡格;伊鹏霏;蒋小林;穆祥
单  位:
北京农学院动物科学技术系;北京农学院兽医学;吉林大学畜牧兽医学院
关键词:
黄芩苷;连翘酯苷;肺微血管内皮细胞;IFN-α;IFN-γ
摘  要:
为了研究黄芩苷、连翘酯苷对大鼠肺微血管内皮细胞(pul monary microvascular endothelial cells,PMVECs)分泌IFN-α、IFN-γ的影响,本试验以体外培养的PMVECs为模型,用ELISA法检测这2种中药成分在不同浓度、不同时间点诱导PMVECs分泌IFN-α和IFN-γ的量。结果表明,黄芩苷在10μg/mL,24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量均最高,连翘酯苷分别在20μg/mL、12 h时和5μg/mL、24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量最高。这一结果为体外筛选诱导PMVECs表达干扰素的中药成分提供了参考,为进一步研究中药成分的抗病毒机理奠定了基础。
译  名:
Effect of Baicalin and Forsythiaside on the Excretion of IFN-α and IFN-γ in Pulmonary Microvascular Endothelial Cells
作  者:
LIN Hong1,HU Ge1,YI Peng-fei2,JIANG Xiao-lin1,MU Xiang3(1.Department of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China)
关键词:
baicalin;forsythiaside;pulmonary microvascular endothelial cells;IFN-α;IFN-γ
摘  要:
To study the effect of baicalin and forsythiaside to induce rat pulmonary microvascular endothelial cells to express IFN-α and IFN-γ,the model was based on cultured PMVECs in vitro.With the method of ELISA,we detected the secretory volume of IFN-α and IFN-γ after PMVECs was induced by baicalin and forsythiaside at different concentration and time.The results indicated that PMVECs produced the most IFN-α and IFN-γ after induced 24 h by baicalin at 10 μg/mL,while forsythiaside was 20 μg/mL at 12 h and 5 μg/mL at 24 h,respectively.The result provided references for screening Chinese herbal medicinal constituents that could induce PMVECs to express IFN,and laid the foundation to further study the anti-virus mechanism of medicinal constituents.

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