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当前位置: 首页 > 文章 > 多粘类芽胞杆菌胞外多糖在微生物农药剂型中的功能研究 农药学学报 2011,13 (6) 52-56
Position: Home > Articles > Function of polysaccharides from Paenibacillus polymyxa in microbial pesticide formulation Chinese Journal of Pesticide Science 2011,13 (6) 52-56

多粘类芽胞杆菌胞外多糖在微生物农药剂型中的功能研究

作  者：
刘振华;魏鸿刚;李元广;鲍柳;李淑兰
单  位：
上海泽元海洋生物技术有限公司;华东理工大学生物反应器工程国家重点实验室
关键词：
多粘类芽胞杆菌;胞外多糖;可湿性粉剂;助悬浮;热保护;紫外保护
摘  要：
为探明生防菌胞外多糖在微生物农药制剂加工中的应用潜力及价值,考察了多糖粗提物对农药载体的助悬浮作用及对生防菌的热保护和紫外保护作用。结果表明:多粘类芽胞杆菌Paenibacillus polymyxa(HY96-2)胞外多糖对硅藻土、高岭土和碳酸钙3种载体具有明显的助悬浮作用,当载体中多糖粗提物添加量为0.25 g/g时,3种载体的悬浮率可分别提高62.6%、161.8%和284.0%。此外,该胞外多糖对HY96-2芽胞还表现出明显的热保护作用,对HY96-2和海洋芽胞杆菌Bacillus marinus(B-9987)的营养细胞及芽胞表现出明显的紫外保护作用:添加质量体积分数为0.5%的多糖粗提物后,HY96-2芽胞在80℃处理20 min后的存活率比对照提高了31.0%,经紫外(UV-B,290～320 nm)照射6 h后,HY96-2营养细胞和芽胞的存活率分别比对照提高了14.1%和22.6%,B-9987营养细胞和芽胞的存活率分别比对照提高了36.4%和29.5%。该胞外多糖可用作多粘类芽胞杆菌可湿性粉剂加工中的助剂,以替代或减少化学助剂的使用。
译  名：
Function of polysaccharides from Paenibacillus polymyxa in microbial pesticide formulation
作  者：
LIU Zhen-hua1,WEI Hong-gang1,LI Yuan-guang*1,BAO Liu1,LI Shu-lan2(1.Institute of Marine Bioprocess Engineering,State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology,Shanghai 200237,China; 2.Shanghai Zeyuan Marine Biotechnology Limited Company,Shanghai 200237,China)
关键词：
Paenibacillus polymyxa;polysaccharides;wettable powder;suspensibility improvement;heat protection;UV protection
摘  要：
In order to search for the potential value of microbial polysaccharide in formulation processing,the functions of Paenibacillus polymyxa polysaccharide crude extract in microbial pesticide were researched with investigation of suspensibility improvement(for pesticide carrier),heat protection and UV protection of P.polymyxa polysaccharide.Results showed that P.polymyxa polysaccharide could improve the carrier suspensibility of diatomite,kaolin and calcium carbonate.When each 1 g of the above three carriers was added 0.25 g polysaccharide crude extract,their suspensibility were increased by 62.6%,161.8% and 284.0%,respectively.Additionally,P.polymyxa polysaccharide showed obvious heat protection for HY96-2 endospores,and obvious ultraviolet(UV) protection for vegetative cells and endospores of both P.polymyxa HY96-2 and Bacillus marinus B-9987.When HY96-2 endospore suspensions were heated at 80 ℃ for 20 min,survival ratio of HY96-2 endospores with 0.5%(W ∶V) polysaccharide addition was increased by 31.0% at the basis of control.When 0.5%(W ∶V) polysaccharide crude extracts were added,survival ratios of HY96-2 vegetative cells and endospores irradiated by UV-B(290-320 nm)for 6 hours were increased by 14.1% and 22.6% compared with the controls,respectively;and survival ratios of vegetative cells and endospores of B-9987 were increased by 36.4% and 29.5% compared with the controls.The polysaccharide extract could be applied as adjuvant in P.polymyxa wettable powder processing progress,taking the place of chemical adjutants.

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