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当前位置: 首页 > 文章 > 黄皮精油对莲雾果实软腐病抑制机理的初探 中国食物与营养 2017,23 (02) 48-51
Position: Home > Articles > Inhibition Mechanism of Essential Oil of Clausena lansium for Soft Rot of Wax-apple Fruits Food and Nutrition in China 2017,23 (02) 48-51

黄皮精油对莲雾果实软腐病抑制机理的初探

作  者：
张史青;钟曼茜;曾凤;从心黎
单  位：
海南大学园艺园林学院
关键词：
莲雾;软腐病;黄皮;精油;抑菌效果
摘  要：
以‘黑金刚’莲雾为试验对象,对莲雾果实采后软腐病病原菌进行了分离纯化,通过从黄皮叶子中提取的精油对软腐病进行抑菌活性测定,研究黄皮精油对菌丝体形态和结构的影响,探讨黄皮精油的抑菌机制。结果表明:不同浓度的黄皮精油对供试的莲雾软腐病均有抑制作用,其中0.6μL/m L浓度下的抑菌率达到60.62%;0.6μL/m L的黄皮精油处理后的菌丝体生长稀疏,分支变少;黄皮精油对病原菌的细胞膜通透性产生了显著影响,可溶性蛋白和还原性糖含量减少,营养物质吸收受阻。综合来看,初步明确了黄皮精油的抗菌机制,其中0.6μL/m L浓度下的抑菌效果最佳。
译  名：
Inhibition Mechanism of Essential Oil of Clausena lansium for Soft Rot of Wax-apple Fruits
作  者：
ZHANG Shi-qing;ZHONG Man-xi;ZENG Feng;CONG Xin-li;College of Horticulture and Landscape,Hainan University;
关键词：
wax apple;;soft rot;;Clausena lansium;;essential oil;;antibacterial effect
摘  要：
Taking ‘Heijingang'wax apples as the test object,the soft rot pathogen has been separated and purified after harvesting the wax apple fruit. Based on the testing result of anti-fungal activity of the essential oil( EO) extracted from the foliage of Clausena lansium against soft rot,we analyzed influence of such essence oil on both the form and structure of mycelia to probe into the antibacterial mechanism of essential oil of Clausena lansium. The results showed all essential oil of Clausena lansium of different concentrations displayed antibacterial effects over the testing soft rot and the inhibition rate of 0. 6 μL / m L EO reached 60. 62%. The mycelium processed with 0. 6 μL / m L EO was sparse with less branches. Essential oils of Clausena lansium had significant impacts over the permeability of pathogenic fungus membrane,which reduced the content of soluble protein and reducing sugar and hindered the fungus' s absorption of nutrients. To conclude,we identified the antibacterial mechanism of essential oils of Clausena lansium preliminarily and found 0. 6 μL / m L EO had the best antibacterial effect.

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