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Modern Journal of Animal Husbandry and Veterinary Medicine
2024
(6)
28-31
LMH贴壁细胞悬浮驯化及病毒增殖研究
作 者：
任培森;吕文静;黄金海;刘海霞;蔡天宁
单 位：
;天津大学生命科学学院
关键词：
LMH贴壁细胞;驯化;悬浮培养;血清4型禽腺病毒(FAdV-4)
摘 要：
研究旨在实现LMH贴壁细胞悬浮培养、稳定传代、高密度生长,并应用于血清4型禽腺病毒(FAdV-4)悬浮培养.研究进行LMH贴壁细胞低含量血清适应驯化,并进行无血清悬浮培养筛选及FAdV-4 RP-4毒株接毒盲传试验.结果显示:LMH悬浮细胞可在无血清培养基中传代第3代后,最终细胞密度达4.00*106cells/mL以上;FAdV-4连续培养至第4代,毒价可达108.13TCID50/0.1 mL.研究表明,用特定培养基可使LMH贴壁细胞实现悬浮驯化,并可应用于FAdV-4稳定增殖.
译 名：
Study on suspension domestication of LMH adherent cells and virus proliferation
单 位：
School of Life Sciences,Tianjin University,Tianjin 300072;Tianjin Ringpu Biotechnology Co.,Ltd.,Tianjin 300381
关键词：
LMH adherent cells%Domestication%Suspension culture%Avian adenovirus type 4(FAdV-4)
摘 要：
The purpose of this study is to realize LMH adherent cell suspension culture,stable passage and high density growth,and apply it to serum avian adenovirus type 4(FAdV-4)suspension culture.In this study,LMH adherent cells were acclimated with low serum content,screened by serum-free suspension culture and blind transmission test of FAdV-4 RP-4 strain.The results showed that LMH suspension cells could be cultured in serum-free medium for the third generation,and the final cell density was over 4.00*106cells/mL.The toxicity of FAdV-4 could reach 108.13TCID50/0.1 mL when cultured to the fourth generation.The results showed that LMH adherent cells could be domesticated in suspension with specific medium,and could be applied to the stable proliferation of FAdV-4.
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