Cloning and Functional Bioinformatics Analysis of Banna Mini-pig Inbred Line Gene LRWD1

ZHANG Xia;WANG Pei;HUO Jinlong;WANG Shuyan;ZHA Xinqin;PAN Weirong;DING Xianqun;CHEN Yuanyuan;Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province,Yunnan Agricultural University;College of Animal Science and Technology,Yunnan Agricultural University;Domestic Animal Breeding and Crossbreed-improvement Station of Yunnan Provice;

leucine-rich repeats(LRR);;WD repeat(WD);;Banna mini-pig inbred line(BMI);;bioinformatics

LRWD1 gene plays an important role in the process of spermatogenesis. Using the LRWD1 mRNA sequences of pig and related species from Gen Bank as reference gene sequences,we designed specific primers and amplified Banna mini-pig inbred line( BMI) LRWD1 gene. Protein sequence was used to carry out functional bioinformatics analysis and a phylogenetic tree was constructed. A coding sequence of 1 944 bp( Gen Bank accession No. KU705639,the corresponding amino acid sequence acces-sion number: AOC89057) of BMI LRWD1 was obtained,which encodes a protein of 647 amino acids,with molecular weight 71. 40 k D,and isoelectric point 7. 52. Functional bioinformatics analysis indicated that the LRWD1 protein contained two conserved domains,with no transmembrane region and signal peptide sequences,and its N-terminal and C-terminal were hydrophobic. This protein has six kinds functional active sites and its 70. 6% located in cytoplasmic. Phylogenetic analysis using LRWD1 sequences demonstrated that BMI had the closest relationship with dog and panda. The results of the study will lay a foundation for further study of the gene about its functions in pig sperm formation.