中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 基于LC-MS/MS技术对CpA感染鸭十二指肠代谢组学分析 经济动物学报 2023,27 (4) 268-274
Position: Home > Articles > Analysis of Duodenal Metabolomics of CpA Infected Ducklings Based on LC-MS/MS Technology Journal of Economic Animal 2023,27 (4) 268-274

基于LC-MS/MS技术对CpA感染鸭十二指肠代谢组学分析

作  者：
王娜;张天天;程振涛;文明;王开功;周碧君
单  位：
关键词：
A型产气荚膜梭菌;鸭;十二指肠;液相色谱-串联质谱(LC-MS/MS);代谢组学
摘  要：
为探讨鸭感染A型产气荚膜梭菌(Clostridium perfringens alpha toxin,CpA)后十二指肠代谢物变化,寻找潜在生物标志物及与致病机制相关的代谢通路,将12只37日龄鸭随机分为对照组和CpA感染组,每组6只,运用液相色谱-串联质谱(LC-MS/MS)技术对对照组和CpA感染90 h的鸭十二指肠进行非靶向代谢组学分析.结果表明:在偏最小二乘判别分析(PLS-DA)模型中,对照组与CpA感染组的得分图呈现明显差异,CpA感染组中部分代谢物发生显著性改变.CpA感染90 h组鉴定出花生四烯酸、3-羟基丁酸、2,3-二羟基苯甲酸、二磷酸腺苷等29种差异代谢物,主要涉及不饱和脂肪酸的生物合成、β-丙氨酸代谢、花生四烯酸代谢、硫代谢、丙酮酸代谢、嘌呤代谢等25条信号通路.CpA感染组与对照组鸭十二指肠之间存在脂类代谢、氨基酸代谢及碳代谢等的差异.研究结果可为CpA感染鸭引起十二指肠损伤机制的研究提供依据.
译  名：
Analysis of Duodenal Metabolomics of CpA Infected Ducklings Based on LC-MS/MS Technology
关键词：
Clostridium perfringens alpha toxin%ducklings%duodenum%liquid chromatography-tandem mass spectrometry(LC-MS/MS)%metabolomics
摘  要：
To investigate the changes of duodenal metabolites of ducks infected with Clostridium perfringens alpha toxin(CpA),and to search for potential biomarkers and metabolic pathways related to the pathogenesis,twelve 37-day-old ducks were randomly divided into control group and CpA infected group with 6 ducks in each group.Liquid chromatoc-tandem mass spectrometry(LC-MS/MS)was used to analyze the non-targeted metabolomics of duodenum in normal group and 90 h CpA infected ducks.The results showed that in partial least squares discriminant analysis(PLS-DA)model,the score maps of control group and CpA infection group showed significant differences,and some metabolites in the CpA infection group had significant changes.After 90 h of CpA infection,29 different metabolites such as arachidonic acid,3-hydroxybutyric acid,2,3-dihydroxybenzoic acid and adenosine diphosphate were identified,and 25 signaling pathways were mainly involved in unsaturated fatty acid biosynthesis,β-alanine metabolism,arachidonic acid metabolism,sulfur metabolism,pyruvate metabolism and purine metabolism.There were differences in lipid metabolism,amino acid metabolism and carbon metabolism of duodenum between CpA infected group and control group.The results of this study could provide a new basis for the study of duodenal injury mechanism caused by CpA infection in ducks.

相似文章

导出引用
计量
文章访问数: 22
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46