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当前位置: 首页 > 文章 > 辐射与杂交结合提高小麦高分子量麦谷蛋白优质亚基转化效果的研究 核农学报 2001,15 (6) 321-326
Position: Home > Articles > EFFECT OF GENE TRANSFORMATION OF WHEAT HMW-GS GENE BY COMBINATION OF CROSS AND IRRADIATION Journal of Nuclear Agricultural Sciences 2001,15 (6) 321-326

辐射与杂交结合提高小麦高分子量麦谷蛋白优质亚基转化效果的研究

作  者：
张宏纪;孙岩;程爱华;吴玉梅
单  位：
黑龙江省农科院谷物分析中心;黑龙江省庆安县农业局;黑龙江省农科院作物育种研究所
关键词：
春小麦;核质互作;核辐射;HMW-GS
摘  要：
本文利用核质互作与辐射处理研究了促进优质HMW GS基因的转化。结果表明 ,不同组合处理间F1籽粒优质亚基 (Glu D15+1 0 )的转化存在差异 ,辐射处理的多以杂合型出现。F2 分离群体中 ,反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本 ,细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率 ,不论是哪种组合 ,反交时 5+1 0亚基的表达频率高于正交 ;无论正交还是反交 ,辐射处理提高了 5+1 0亚基的表达频率 ,而且在不同组合间存在差异。
译  名：
EFFECT OF GENE TRANSFORMATION OF WHEAT HMW-GS GENE BY COMBINATION OF CROSS AND IRRADIATION
作  者：
ZHANG Hong\|ji 1\ SUN Yan 1\ CHENG Ai\|hua 2\ WU Yu\|mei 3 (1 Crop Breeding Institute of Heilongjiang Academy of Agricultural Science, Harbin Heilongjiang prov.\ 150086; 2 Center of Cereal Quality, Heilongjiang Academy of Agricultural Sciences, Ha
关键词：
spring wheat; interaction of nuclear and cytoplasm; irradiation; HMW\|GS gene
摘  要：
Irradiation treatment and the interaction of nuclear and cytoplasm were employed to promote the transformation of HMW\|GS gene with high wheat quality. The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

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