Position: Home > Articles > 荧光定量分析猪睾丸组织中miR-34b的表达规律
Chinese Journal of Veterinary Science
2013,33
(5)
785-787,794
荧光定量分析猪睾丸组织中miR-34b的表达规律
作 者：
赵伟;李传民;高峰;张晓军;王大力;白春燕;孙博兴
单 位：
吉林大学畜牧兽医学院
关键词：
miRNA-34b;军牧1号白猪;荧光定量
摘 要：
运用荧光定量分析方法检测军牧1号白猪不同发育阶段睾丸组织miRNA-34b的表达量差异,结果:miRNA-34b在3月龄前的表达量都非常低,从5月龄开始表达量呈明显上升,7月龄达到峰值后开始明显下降.在猪的发育过程中,miRNA-34b的表达趋势曲线呈先增后降.结果表明miRNA-34b可能与猪雄性生殖发育相关.
单 位：
College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China
关键词：
miRNA-34b%Junmu No.1 white pig%real time quantitative PCR
摘 要：
MicroRNAs(miRNAs) are short(-22 nucleotides) non-coding RNA molecules that play important roles by repressing translation or cleaving RNA transcripts.Some reports showed miRNA-34b is an important regulator in germ cell differentiation process of male humans and mice.We detected the different expressions of miR-34b of testicular tissue by real time quantitative PCR(RT-PCR) in Junmu No.1 white pig among the whole developmental stages.The results showed the expressions of miRNA-34b keep very low level before 3-month-old,rising dramatically from 5-month-old,reaching a peak at 7-month-old,and then descend apparently.Overview the expression trend of miRNA-34b in the whole sexual development processes,it runs a rule that first rising and then descending.The conclusion is anticipated that miR-34b keep a high possibility correlation with sexual development of male pig.
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