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Position: Home > Articles > Analysis and application of transferable EST-SSR markers from Stylosanthes to Desmodium Pratacultural Science 2016,33 (11) 2237-2247

柱花草EST-SSR标记在山蚂蝗中的可转移性与应用

作  者:
罗小燕;张瑞花;贾庆麟;王文强;白昌军;丁西朋
单  位:
中国热带农业科学院热带作物品种资源研究所
关键词:
山蚂蝗;柱花草;EST-SSR标记;可转移性;亲缘关系分析
摘  要:
为鉴定山蚂蝗(Desmodium)种质资源的遗传背景及亲缘关系,选取92对柱花草(Stylosanthes spp.)EST-SSR标记和来自8个种的16份山蚂蝗材料,分析柱花草EST-SSR标记在山蚂蝗中的通用性,并对8种山蚂蝗进行亲缘关系分析。研究结果表明,柱花草EST-SSR标记在8种山蚂蝗种的可转移率为63.04%~73.91%,其中50对可在全部8种山蚂蝗中进行有效扩增。16对多态性EST-SSR标记在16份山蚂蝗种质中共检测到等位基因35个,平均2.19个。聚类分析表明,16份山蚂蝗种质可分为5类,与形态学划分结果相似。可见,柱花草EST-SSR标记在山蚂蝗上具有较高的可转移性,可应用于山蚂蝗种质资源评价及亲缘关系研究。
译  名:
Analysis and application of transferable EST-SSR markers from Stylosanthes to Desmodium
作  者:
Luo Xiao-yan;Zhang Rui-hua;Jia Qing-lin;Wang Wen-qiang;Bai Chang-jun;Ding Xi-peng;Institute of Tropical Crops Genetic Resources,Chinese Academy of Tropical Agricultural Sciences;
关键词:
Desmodium;;Stylosanthes;;EST-SSR marker;;transferability;;genetic relationship analysis
摘  要:
To identify genetic background and relationships of Desmodium varieties,92 Stylosanthes EST-SSR markers and 16 varieties from 8 Desmodiumspecies were used to assess the transferability of Stylosanthes ESTSSR markers in Desmodiumgenus and analyze the genetic relationships among 8 Desmodiumspecies.The results indicated that the transferability of Stylosanthes EST-SSR markers across 8 Desmodium species ranged from 63.04%to 73.91%,and 50 markers of them could produce reliable amplification in all 8 Desmodiumspecies.A total of 35 alleles were generated by 16 polymorphic markers with an average of 2.19 among 16 Desmodiumvarieties.Cluster analysis showed that 16 Desmodiumvarieties can be divided into 5clusters,which was similar to the classification based on morphology.Therefore,Stylosanthes EST-SSR markers showed high transferability in Desmodium,and it would be suitable for germplasm evaluation and genetic relationships analysis of Desmodium.

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