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网室内家蝇和红头丽蝇为洋葱雄性不育系授粉效果的比较

作  者:
冉东生;冯秋影;王一丹;崔成日;徐启江
关键词:
洋葱;细胞质雄性不育系;授粉;家蝇;红头丽蝇
摘  要:
以洋葱细胞质雄性不育系'MS2016A'和自交系'T609C'为试材,采用家蝇、红头丽蝇、人工和自然授粉方式,研究了网室内洋葱雄性不育系授粉效果,以期为提高洋葱F1种子产量和品质提供参考.结果表明:2种苍蝇的授粉行为相似,工作时长没有显著区别,在网室内的活动时间大约为7h,与洋葱小花开放时间基本吻合,单花停留时间为2~3s.与自然授粉相比较,苍蝇授粉显著提高杂交洋葱母本的结籽率、千粒质量和发芽率.与人工授粉相比较,苍蝇授粉仅显著提高结实率,千粒质量和发芽率无显著差异.家蝇与红头丽蝇授粉后的3项指标分别为80.98%、4.41g、88.43%和78.22%、4.45g、87.94%,差异不显著.家蝇传粉效率略优于红头丽蝇.
作  者:
RAN Dongsheng;FENG Qiuying;WANG Yidan;CUI Chengri;XU Qijiang;Heilongjiang Foreign Cooperation Center for Science and Technology;College of Life Sciences,Northeast Forestry University;Harbin Allium Agricultural Science and Technology Co.Ltd.;
关键词:
onion(Allium cepa L.);;cytoplasmic male sterile line;;pollination;;housefly(Musca domestica);;blowfly(Calliphora vicina)
摘  要:
The Cytoplasmic male sterile line ‘MS2016 A’ and inbred line ‘T609 C’ of onion(Allium cepa L.)were taken as the test materials,the effects of different pollination methods on the production and quality of onion F1 seed in cages were studied,to provide reference for solving the problem of inadequate artificial pollination and low hybrid seed yields of caged onion.The pollination efficiencies of different pollination methods on caged onion were compared.Four pollination methods were applied as houseflies(Musca domestica)pollination,blowflies(Calliphora vicina)pollination,artificial pollination,and natural pollination.The results showed that the pollination behavior of two fly species,Musca domestica and Calliphora vicina,were similar.There was no significant difference in daily working time between two fly species.The activity time in net-house was 7 hours,which was coincident with the time of the onion florets flowering,while the single flower retention time was 2 to3 seconds.Compared with that of natural pollination,the seed setting rate per umbel,1 000-seed weight,and the germination rate of flies pollination increased significantly.The seed setting rate of umbel from artificial pollination were significantly less than those from the fly-pollination,but there was no significant difference in the germination rate and 1 000-seed weight between artificial and flies pollination.The seed-setting rate per umbel,1 000-seed weight,and the germination rate of caged onion cytoplasmic male sterile lines pollinated by houseflies and blowflies were 80.98%,4.41 g,88.43% and 78.22%,4.45 g,87.94%,respectively,which were no significant difference with each other.Housefly was a little bit better pollinator of caged onion than blowfly.

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