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当前位置: 首页 > 文章 > 一株基因Ⅵ型鸭新城疫病毒F基因的克隆及序列分析 中国畜牧兽医 2010,37 (5) 92-98
Position: Home > Articles > Cloning and Sequence Analysis of F Gene of a Genetype Ⅵ Newcastle Disease Virus Isolated from Duck China Animal Husbandry & Veterinary Medicine 2010,37 (5) 92-98

一株基因Ⅵ型鸭新城疫病毒F基因的克隆及序列分析

作  者：
蔡丽丽;吴志新;王俊峰;黄兴国;廖明;任涛;黄淑坚
单  位：
华南农业大学兽医学院;佛山科学技术学院生命科学学院
关键词：
鸭源新城疫病毒;F基因;克隆;序列分析
摘  要：
以一株鸭新城疫病毒NDV-SS分离株基因组RNA为模板,采用RT-PCR方法扩增其F基因,并进行克隆和序列测定。通过序列分析软件将该毒株F基因氨基酸推导序列与GenBank上已发表的新城疫病毒(Newcastle disease virus,NDV)F基因相关代表序列进行同源性及遗传进化关系分析。测序结果表明,NDV-SS株F基因全长1662bp,编码553个氨基酸,其蛋白裂解位点氨基酸序列为112R-R-Q-K-R-F117,符合NDV强毒株特有的序列结构特征。同源性及遗传进化关系分析结果表明,NDV-SS株为基因Ⅵ型毒株,与鸽源新城疫IT-227、Italy-2736分离株具有较高同源性和较近的亲缘关系,推测其可能是由鸽源新城疫病毒变异所产生的。
译  名：
Cloning and Sequence Analysis of F Gene of a Genetype Ⅵ Newcastle Disease Virus Isolated from Duck
作  者：
CAI Li-li1,WU Zhi-xin1,WANG Jun-feng1,HUANG Xing-guo2,LIAO Ming1,REN Tao1,HUANG Shu-jian2(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2.College of Life Science,Foshan University,Foshan 528231,China)
关键词：
Newcastle disease virus; ducks; F gene; cloning; sequence analysis
摘  要：
Using the genome RNA of NDV-SS strain of Newcastle disease virus (NDV) isolated from duck as a template,the F gene of NDV-SS strain was amplified by reverse transcription polymerase chain reaction (RT-PCR). Then its gene cloning was completed,and its nucleotide sequence was determined. Nucleotide sequence homology and evolutionary processes analyses on amino acids sequence of F gene between SS strain and other NDV reference strains published in GenBank was executed using the sequence analysis soft. The F gene sequence of SS strain showed that NDV-SS strain was determined with its full-length of 1662 bp coding 553 amino acids. Amino acids sequence of F protein proteolytic cleavage position was 112R-R-Q-K-R-F117,indicated it is the virulent NDV strain.And the results of nucleotide sequence homology and evolutionary processes analyses showed that this strain belonged to the gene type Ⅵ,and had a close relationship with IT-227 and Italy-2736 strain. The presumable result was that this strain evolved from the NDV of pigeon origin.

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